Alcaraz M J, Habib A, Créminon C, Vicente A M, Lebret M, Lévy-Toledano S, Maclouf J
Department of Pharmacology, University of Valencia, Spain.
Biochim Biophys Acta. 2001 Apr 3;1526(1):13-6. doi: 10.1016/s0304-4165(01)00112-x.
Unstimulated RAW 264.7 macrophages express negligible heme oxygenase-1 (HO-1) protein but incubation with the nitric oxide (NO) donor spermine nonoate (SPNO) induced HO-1 and weakly cyclo-oxygenase-2 (COX-2) protein. This effect was potentiated by coincubation with the COX-2 selective inhibitor, SC58125. Cells incubated with SPNO showed a strong increase in HO-1 mRNA levels after 4 h with a significant potentiation in the presence of SC58125, which did not modify HO-1 mRNA stability. The induction of HO-1 by NO and its potentiation by anti-inflammatory agents may play a role in inflammatory and immune responses.
未刺激的RAW 264.7巨噬细胞表达的血红素加氧酶-1（HO-1）蛋白可忽略不计，但与一氧化氮（NO）供体精胺亚硝基铁氰化物（SPNO）孵育可诱导HO-1和弱诱导环氧化酶-2（COX-2）蛋白。与COX-2选择性抑制剂SC58125共同孵育可增强这种效应。用SPNO孵育的细胞在4小时后HO-1 mRNA水平显著升高，在存在SC58125的情况下有明显增强，而SC58125并不改变HO-1 mRNA的稳定性。NO诱导HO-1及其被抗炎剂增强的作用可能在炎症和免疫反应中发挥作用。
