气体递质的相互作用有助于调节早产儿的微血管张力（失调）。
Interactions of the gasotransmitters contribute to microvascular tone (dys)regulation in the preterm neonate.
作者信息
Dyson Rebecca M, Palliser Hannah K, Latter Joanna L, Kelly Megan A, Chwatko Grazyna, Glowacki Rafal, Wright Ian M R
机构信息
Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, NSW, 2305, Australia; School of Medicine and Public Health, University of Newcastle, Callaghan, NSW, 2308, Australia; Illawarra Health and Medical Research Institute and Graduate School of Medicine, University of Wollongong, NSW, 2522, Australia.
Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, NSW, 2305, Australia; School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, 2308, Australia.
出版信息
PLoS One. 2015 Mar 25;10(3):e0121621. doi: 10.1371/journal.pone.0121621. eCollection 2015.
BACKGROUND & AIMS: Hydrogen sulphide (H2S), nitric oxide (NO), and carbon monoxide (CO) are involved in transitional microvascular tone dysregulation in the preterm infant; however there is conflicting evidence on the interaction of these gasotransmitters, and their overall contribution to the microcirculation in newborns is not known. The aim of this study was to measure the levels of all 3 gasotransmitters, characterise their interrelationships and elucidate their combined effects on microvascular blood flow.
METHODS
90 preterm neonates were studied at 24h postnatal age. Microvascular studies were performed by laser Doppler. Arterial COHb levels (a measure of CO) were determined through co-oximetry. NO was measured as nitrate and nitrite in urine. H2S was measured as thiosulphate by liquid chromatography. Relationships between levels of the gasotransmitters and microvascular blood flow were assessed through partial correlation controlling for the influence of gestational age. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow and derive a theoretical model of their interactions.
RESULTS
No relationship was observed between NO and CO (p = 0.18, r = 0.18). A positive relationship between NO and H2S (p = 0.008, r = 0.28) and an inverse relationship between CO and H2S (p = 0.01, r = -0.33) exists. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow. The model with the best fit is presented.
CONCLUSIONS
The relationships between NO and H2S, and CO and H2S may be of importance in the preterm newborn, particularly as NO levels in males are associated with higher H2S levels and higher microvascular blood flow and CO in females appears to convey protection against vascular dysregulation. Here we present a theoretical model of these interactions and their overall effects on microvascular flow in the preterm newborn, upon which future mechanistic studies may be based.
背景与目的
硫化氢（H₂S）、一氧化氮（NO）和一氧化碳（CO）参与早产儿过渡性微血管张力调节异常；然而，关于这些气体信号分子之间的相互作用存在相互矛盾的证据，并且它们对新生儿微循环的总体贡献尚不清楚。本研究的目的是测量所有三种气体信号分子的水平，表征它们之间的相互关系，并阐明它们对微血管血流的综合影响。
方法
对90例出生后24小时的早产儿进行研究。通过激光多普勒进行微血管研究。通过多波长血氧计测定动脉碳氧血红蛋白水平（CO的一种测量指标）。尿液中的硝酸盐和亚硝酸盐作为NO进行测量。通过液相色谱法将H₂S测量为硫代硫酸盐。通过控制胎龄影响的偏相关分析评估气体信号分子水平与微血管血流之间的关系。使用结构方程模型来检验这些对微血管血流的影响组合，并推导它们相互作用的理论模型。
结果
未观察到NO与CO之间存在关联（p = 0.18，r = 0.18）。NO与H₂S之间存在正相关（p = 0.008，r = 0.28），CO与H₂S之间存在负相关（p = 0.01，r = -0.33）。使用结构方程模型来检验这些对微血管血流的影响组合。呈现了拟合度最佳的模型。
结论
NO与H₂S之间以及CO与H₂S之间的关系在早产新生儿中可能具有重要意义，特别是因为男性的NO水平与较高的H₂S水平和较高的微血管血流相关，而女性的CO似乎对血管调节异常具有保护作用。在此，我们提出了这些相互作用及其对早产新生儿微血管血流的总体影响的理论模型，未来的机制研究可能基于此。
Zotero 插件