Casanova A, Obreque J, Sandino A M, Jashés M
Laboratorio de Virología, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Alameda 3363, Santiago-Chile, Casilla 40 Correo 33, Santiago-Chile, Chile.
FEMS Microbiol Lett. 2001 Apr 1;197(1):19-22. doi: 10.1111/j.1574-6968.2001.tb10576.x.
Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR using L1 and G1 primers, differing from that described where only one ITS region was found. By Southern blot, it was established that these two amplification products contained sequences related to P. salmonis ITS. Sequence analysis confirmed that P. salmonis had two ITS regions: ITS A and ITS B. In both isolates, the smaller (ITS B) corresponded to ITS sequences previously described for each one, and the larger (ITS A) were almost the same as their respective ITS B sequences interrupted by an insert which contained two tRNAs genes: tRNA-Ile and tRNA-Ala.
鲑鱼立克次氏体是鲑鱼立克次氏体败血症的病原体，该疾病影响鲑鱼养殖业。我们分析了智利鲑鱼立克次氏体分离株LF-89和EM-90的16S-23S rDNA间隔区（内部转录间隔区，ITS）。使用L1和G1引物通过PCR获得了两个主要的ITS扩增产物，这与仅发现一个ITS区域的描述不同。通过Southern杂交，确定这两个扩增产物包含与鲑鱼立克次氏体ITS相关的序列。序列分析证实鲑鱼立克次氏体有两个ITS区域：ITS A和ITS B。在两个分离株中，较小的（ITS B）与先前为每个分离株描述的ITS序列相对应，而较大的（ITS A）几乎与它们各自被一个包含两个tRNA基因（tRNA-Ile和tRNA-Ala）的插入片段中断的ITS B序列相同。
