Clarke S N, Koh M T, Bernstein I L
Department of Psychology, University of Washington, Seattle 98195, USA.
Chem Senses. 2001 Mar;26(3):253-7. doi: 10.1093/chemse/26.3.253.
Adult Fischer 344 (F344) rats fail to display any preference for NaCl solutions at concentrations typically preferred by other rat strains. To determine whether this behavior is due to a strain difference in NaCl detection threshold, a conditioned taste aversion (CTA) was first established to a suprathreshold concentration of NaCl (0.1 M). Then, a series of dilute NaCl solutions, ranging from 0.0 to 0.011 M NaCl, were presented to F344 (n = 16) and Wistar (n = 16) rats. The lowest concentration at which there was a reliable difference in the preference scores of conditioned and control rats was defined as the detection threshold. Results indicate that the detection threshold for NaCl lies between 0.001 and 0.002 M NaCl for both F344 and Wistar rats. The addition of the sodium channel blocker amiloride to the NaCl solutions raised the detection threshold 10-fold to 0.03-0.04 M NaCl for both strains of rats. These results suggest that the NaCl detection thresholds of F344 and Wistar rats are similar and that these strains do not differ in the degree to which amiloride raises this threshold.
成年Fischer 344（F344）大鼠对氯化钠溶液不会表现出任何偏好，而其他品系的大鼠通常更喜欢这种浓度的溶液。为了确定这种行为是否是由于氯化钠检测阈值的品系差异所致，首先对超阈值浓度的氯化钠（0.1 M）建立了条件性味觉厌恶（CTA）。然后，向F344（n = 16）和Wistar（n = 16）大鼠提供一系列稀释的氯化钠溶液，浓度范围为0.0至0.011 M氯化钠。条件大鼠和对照大鼠的偏好分数存在可靠差异的最低浓度被定义为检测阈值。结果表明，F344和Wistar大鼠的氯化钠检测阈值均在0.001至0.002 M氯化钠之间。向氯化钠溶液中添加钠通道阻滞剂阿米洛利后，两种品系大鼠的检测阈值均提高了10倍，达到0.03 - 0.04 M氯化钠。这些结果表明，F344和Wistar大鼠的氯化钠检测阈值相似，并且这两个品系在阿米洛利提高该阈值的程度上没有差异。
