Jaborek-Hugo S, von Holst C, Allen R, Stewart T, Willey J, Anklam E
European Commission, DG Joint Research Centre, Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit, Ispra, Italy.
Food Addit Contam. 2001 Feb;18(2):121-7. doi: 10.1080/02652030010021422.
In this paper we describe the validation and utilization of the Hybrinzme DELFIA immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimized to detect concentrations of PCBs in animal fat at or above 200 ng/g. The limit of detection of the assay was less than 50 ng/g. When the action level for the DELFIA assay was set at 100 ng/g to detect samples having 200 ng/g or more PCBs, the false negative rate was estimated at 0.2%. The false positive rate depends on the concentration of PCBs in the sample population and was estimated at 4% and 7% for samples having 10 ng/g and 20 ng/g PCBs, respectively. By utilizing the DELFIA assay system to select for positive samples and GC/MS analysis for confirmation analysis, an accurate and cost-effective programme has been established for the high throughput detection of PCBs in animal products.
在本文中，我们描述了用于检测动物脂肪中多氯联苯（PCBs）的Hybrinzme DELFIA免疫分析方法的验证和应用。该免疫分析方法采用了一种快速样品处理方案，该方案已被优化，用于检测动物脂肪中浓度为200 ng/g及以上的多氯联苯。该分析方法的检测限小于50 ng/g。当将DELFIA分析方法的行动水平设定为100 ng/g以检测多氯联苯含量为200 ng/g或更高的样品时，假阴性率估计为0.2%。假阳性率取决于样品群体中多氯联苯的浓度，对于多氯联苯含量为10 ng/g和20 ng/g的样品，假阳性率分别估计为4%和7%。通过利用DELFIA分析系统筛选阳性样品，并使用气相色谱/质谱（GC/MS）分析进行确证分析，已建立了一个准确且具有成本效益的方案，用于动物产品中多氯联苯的高通量检测。
