Yücel F, Cirakoğlu B, Mustafaeva Z, Mustafaev M
Institute for Genetic Engineering and Biotechnology, TUBITAK Marmara Research Center, Kocaeli, Turkey.
Hybridoma. 2001 Feb;20(1):11-5. doi: 10.1089/027245701300060346.
Cu2+-mediated complex formation between copolymers of acrylic acid with N-isopropyl-acyrlamide (CP1) and negatively charged covalent conjugate of bovine serum albumin with progesterone (BSA.P) was studied in neutral water in the presence of Cu2+. It was shown that under conditions where CP and BSA.P are negatively charged and incapable of binding to one another, the divalent Cu2+ act as "fasteners" promoting the formation of relatively stable water-soluble ternary polycomplexes. The immunogenic properties of ternary mixtures BSA.P-Cu2+-CP1 and BSA.P+IFA were investigated and the production of monoclonal antibodies (MAbs) against progesterone hormone was analyzed. Fusion following the two different immunization procedures resulted in the growth of comparable numbers of progesterone-specific MAbs with apparently similar antigen affinities. Thus, immunizations using antigens in BSA.P-Cu2+-CP1 appear to provide an efficient alternative to incomplete Freund's adjuvant.
研究了在中性水中，在Cu2+存在的情况下，丙烯酸与N-异丙基丙烯酰胺的共聚物（CP1）与牛血清白蛋白与孕酮的带负电荷共价共轭物（BSA.P）之间由Cu2+介导的复合物形成。结果表明，在CP和BSA.P带负电荷且彼此无法结合的条件下，二价Cu2+充当“紧固件”，促进形成相对稳定的水溶性三元多聚体。研究了三元混合物BSA.P-Cu2+-CP1和BSA.P+IFA的免疫原性，并分析了抗孕酮激素单克隆抗体（MAbs）的产生。两种不同免疫程序后的融合导致产生了数量相当的具有明显相似抗原亲和力的孕酮特异性MAbs。因此，使用BSA.P-Cu2+-CP1中的抗原进行免疫似乎为不完全弗氏佐剂提供了一种有效的替代方法。
