Mustafaev M, Yücel F, Cirakoğlu B, Bermek E
Immunol Lett. 1996 Sep;52(2-3):63-8. doi: 10.1016/0165-2478(96)02582-5.
The immunogenic properties of water soluble (PAA-Cu(2+)-BSA) and colloidal (PAA-Cu(2+)-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity. A comparative study of immunogenic properties of polycomplexes versus BSA.P + incomplete Freund's adjuvant (IFA) mixtures revealed differences in regards to the specificity of antibody production. In contrast to the IFA system, polycomplexes were able to generate P- as well as BSA-specific antibodies. Such a response is determined, possibly, by increases in the immunogenicity of weak antigenic determinants on the surface of protein globules and or by the representation of dormant determinants existing in the miner site upon complex formation with polyelectrolytes. Finally, using a short immunization procedure based on use of PAA-Cu(2+)-BSA polycomplexes, we produced seven monoclonal antibodies against progesterone included in polyelectrolyte complexes with affinities Kd ranging between 1.3 x 10 (-5) and 9 x 10(-8) M.
研究了水溶性（PAA-Cu(2+)-BSA）和胶体（PAA-Cu(2+)-BSA.P）多聚复合物的免疫原性，并分析了所产生抗体的特异性。含有孕酮的多聚复合物似乎具有高度的类固醇特异性免疫原活性。对多聚复合物与BSA.P+不完全弗氏佐剂（IFA）混合物的免疫原性进行的比较研究揭示了抗体产生特异性方面的差异。与IFA系统不同，多聚复合物能够产生P特异性以及BSA特异性抗体。这种反应可能是由蛋白质球体表面弱抗原决定簇免疫原性的增加和/或与聚电解质形成复合物时存在于微小位点的休眠决定簇的呈现所决定的。最后，使用基于PAA-Cu(2+)-BSA多聚复合物的短期免疫程序，我们制备了七种针对包含在聚电解质复合物中的孕酮的单克隆抗体，其亲和力Kd在1.3×10^(-5)至9×10^(-8) M之间。
