Alaiya A A, Oppermann M, Langridge J, Roblick U, Egevad L, Brindstedt S, Hellström M, Linder S, Bergman T, Jörnvall H, Auer G
Department of Oncology and Pathology, Karolinska Institutet and Hospital, Stockholm, Sweden.
Cell Mol Life Sci. 2001 Feb;58(2):307-11. doi: 10.1007/pl00000858.
Protein patterns in cells collected from benign prostatic tissues and prostate carcinomas were analyzed using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Polypeptide expression was evaluated by computer-assisted image analysis (PDQUEST). Proteins expressed by prostate tumors were identified via in-gel digestion and subsequent matrix-assisted laser desorption/ionization mass spectrometry. In addition to cytoskeletal and mitochondrial proteins, a 40-kDa protein was identified as prostatic acid phosphatase (PAP). PAP expression decreased approximately twofold between benign and malignant tissue. Increased expression of heat shock protein 70 and decreased expression of tropomyosin 1 were also observed in the malignant tissue. The analysis of prostate material by two-dimensional gel electrophoresis and mass spectrometry shows that particular proteins are of interest as markers of disease.
使用二维聚丙烯酰胺凝胶电泳和质谱分析法对从良性前列腺组织和前列腺癌中收集的细胞中的蛋白质模式进行了分析。通过计算机辅助图像分析（PDQUEST）评估多肽表达。通过凝胶内消化和随后的基质辅助激光解吸/电离质谱法鉴定前列腺肿瘤表达的蛋白质。除细胞骨架和线粒体蛋白外，一种40 kDa的蛋白质被鉴定为前列腺酸性磷酸酶（PAP）。PAP表达在良性和恶性组织之间下降了约两倍。在恶性组织中还观察到热休克蛋白70表达增加和原肌球蛋白1表达减少。通过二维凝胶电泳和质谱分析法对前列腺材料进行分析表明，特定蛋白质作为疾病标志物具有重要意义。
