Burmeister J J, Gerhardt G A
Department of Anatomy, Center for Sensor Technology, University of Kentucky, Lexington 40536-0098, USA.
Anal Chem. 2001 Mar 1;73(5):1037-42. doi: 10.1021/ac0010429.
A self-referencing technique utilizing two microelectrodes on a ceramic-based multisite array is employed for confirmation and elimination of interferences detected by enzyme-based microelectrodes. The measurement of L-glutamate using glutamate oxidase was the test system; however, other oxidase enzymes such as glucose oxidase can be employed. One recording site was coated with Nafion with L-glutamate oxidase and bovine serum albumin (BSA) cross-linked with glutaraldehyde while the other had Nafion with BSA cross-linked with glutaraldehyde. Differences in the chemistry of the two recording sites allowed for identification and elimination of interfering signals to be removed from the analyte response. The electrode showed low detection limits (LOD = 0.98 +/- 0.09 microM, signal-to-noise ratio of 3), fast response times (T90 approximately 1 s), and excellent linearity (R2 = 0.999 +/- 0.000) over the concentration range of 0-200 microM for calibrations of L-glutamate in vitro. The selectivity and dimensions of the multisite electrode allow in vivo glutamate measurements. This electrode has been applied to in vivo measurements of the clearance of locally applied glutamate and release of glutamate in the prefrontal cortex of anesthetized rats. In addition, a aimilar approach has been applied to the development of a microelectrode for measures of glucose.
一种基于陶瓷的多部位阵列上利用两个微电极的自参考技术，用于确认和消除基于酶的微电极检测到的干扰。使用谷氨酸氧化酶测量L-谷氨酸是测试系统；然而，也可以使用其他氧化酶，如葡萄糖氧化酶。一个记录部位涂有与戊二醛交联的L-谷氨酸氧化酶和牛血清白蛋白（BSA）的Nafion，而另一个涂有与戊二醛交联的BSA的Nafion。两个记录部位化学性质的差异使得能够识别并消除分析物响应中的干扰信号。该电极在体外L-谷氨酸校准的0-200 microM浓度范围内显示出低检测限（LOD = 0.98 +/- 0.09 microM，信噪比为3）、快速响应时间（T90约为1秒）和出色的线性（R2 = 0.999 +/- 0.000）。多部位电极的选择性和尺寸允许进行体内谷氨酸测量。该电极已应用于麻醉大鼠前额叶皮层中局部应用谷氨酸的清除和谷氨酸释放的体内测量。此外，类似的方法已应用于开发用于测量葡萄糖的微电极。
