Huang W H, Pang D W, Tong H, Wang Z L, Cheng J K
Department of Chemistry, Wuhan University, China.
Anal Chem. 2001 Mar 1;73(5):1048-52. doi: 10.1021/ac0008183.
A new and facile method has been developed for the fabrication of low-noise carbon fiber microelectrodes (CFMEs) and carbon fiber nanoelectrodes (CFNEs). The carbon fiber was flame-fuse sealed in the tip of the glass capillary. The CFMEs were made by cutting the protruding carbon fiber to the desired length, and the CFNEs were achieved by etching the protruding carbon on the flame to form a nanometer-scale tip. The tip of CFNEs can be controlled within the range from 100 to 300 nm. Thus, no epoxy wax was involved in the CFMEs and CFNEs. The experimental results of inspecting CFMEs and CFNEs by scanning electron microscopy demonstrated that the surface of the electrodes and the glass/fiber interface are very smooth. Therefore, the noise caused by the glass/fiber of these electrodes is much lower than that of the electrodes fabricated conventionlly. The electrodes were characterized by ferricyanide, catecholamine (dopamine,DA), norepinephrine (NE), and epinephrine (E)) and 5-hydroxytryptamine (5-HT) neurotransmitters using CV, LSV, DPV, and FSCV. The results showed that the CFMEs and CFNEs have very excellent electrochemical behavior and high sensitivity. The CV and DPV detection limits of DA, NE, and E are 7.6 x 10(-8), 7.0 x 10(-8), and 5.0 x 10(-8) mol/L, and the DPV detection limits of DA, NE, and E are 4.0 x 10(-8), 1.0 x 10(-7), and 2.2 x 10(-7) mol/L, respectively. This experiment offers a new and facile method for the fabrication of CFMEs and CFNEs of very high sensitivity and low noise.
已开发出一种新颖且简便的方法来制备低噪声碳纤维微电极（CFMEs）和碳纤维纳米电极（CFNEs）。将碳纤维火焰熔封在玻璃毛细管尖端。通过将突出的碳纤维切割至所需长度制成CFMEs，通过蚀刻火焰上突出的碳形成纳米级尖端来制备CFNEs。CFNEs的尖端可控制在100至300纳米范围内。因此，CFMEs和CFNEs的制备过程中不涉及环氧蜡。通过扫描电子显微镜检查CFMEs和CFNEs的实验结果表明，电极表面和玻璃/纤维界面非常光滑。因此，这些电极的玻璃/纤维所产生的噪声远低于传统制备的电极。使用循环伏安法（CV）、线性扫描伏安法（LSV）、差分脉冲伏安法（DPV）和快速扫描循环伏安法（FSCV）对电极进行铁氰化物、儿茶酚胺（多巴胺，DA）、去甲肾上腺素（NE）、肾上腺素（E）和5-羟色胺（5-HT）神经递质表征。结果表明，CFMEs和CFNEs具有非常优异的电化学行为和高灵敏度。DA、NE和E的CV和DPV检测限分别为7.6×10⁻⁸、7.0×10⁻⁸和5.0×10⁻⁸mol/L，DA、NE和E的DPV检测限分别为4.0×10⁻⁸、1.0×10⁻⁷和2.2×10⁻⁷mol/L。该实验为制备具有非常高灵敏度和低噪声的CFMEs和CFNEs提供了一种新颖且简便的方法。
