Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Anal Chem. 2012 Mar 20;84(6):2949-54. doi: 10.1021/ac3000368. Epub 2012 Mar 6.
Fabrication of carbon microelectrode arrays, with up to 15 electrodes in total tips as small as 10-50 μm, is presented. The support structures of microelectrodes were obtained by pulling multiple quartz capillaries together to form hollow capillary arrays before carbon deposition. Carbon ring microelectrodes were deposited by pyrolysis of acetylene in the lumen of these quartz capillary arrays. Each carbon deposited array tip was filled with epoxy, followed by beveling of the tip of the array to form a deposited carbon-ring microelectrode array (CRMA). Both the number of the microelectrodes in the array and the tip size are independently tunable. These CRMAs have been characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, and electrogenerated chemiluminescence. Additionally, the electrochemical properties were investigated with steady-state voltammetry. In order to demonstrate the utility of these fabricated microelectrodes in neurochemistry, CRMAs containing eight microring electrodes were used for electrochemical monitoring of exocytotic events from single PC12 cells. Subcellular temporal heterogeneities in exocytosis (i.e. cold spots vs hot spots) were successfully detected with the CRMAs.
本文介绍了一种总共有 15 个电极的碳微电极阵列的制作方法，其尖端小至 10-50μm。通过将多个石英毛细管拉在一起，在碳沉积之前形成空心毛细管阵列，从而获得微电极的支撑结构。在这些石英毛细管阵列的管腔中，通过乙炔的热解沉积碳环微电极。将每个沉积的碳阵列尖端用环氧树脂填充，然后将阵列尖端斜切，形成沉积碳环微电极阵列（CRMA）。阵列中的微电极数量和尖端尺寸均可独立调节。使用扫描电子显微镜、能量色散 X 射线光谱和电致化学发光对这些 CRMA 进行了表征。此外，还通过稳态伏安法研究了其电化学性质。为了证明这些制备的微电极在神经化学中的实用性，使用含有 8 个微环电极的 CRMA 对单个 PC12 细胞的胞吐事件进行电化学监测。成功地用 CRMA 检测到胞吐作用中的亚细胞时间异质性（即冷点与热点）。
