Abd-Alla M D, Wahib A A, Ravdin J I
El-Hussein University Hospital, Cairo, Egypt.
Am J Trop Med Hyg. 2000 May;62(5):579-82. doi: 10.4269/ajtmh.2000.62.579.
We performed a prospective field study in the village of Kafer Daoud in Menofia, Egypt to compare the fecal culture method with enzyme linked immuno assay (ELISA) for detection of 170 kDa lectin antigen in feces for diagnosis of asymptomatic Entamoeba histolytica and Entamoeba dispar infection. All subjects with E. histolytica or E. dispar infection detected by culture also had positive ELISA for amebic antigen in their feces and an additional 57 Entameoba infections missed by culture were detected by ELISA (P < 0.001 compared to culture). The presence of fecal anti-lectin IgA antibodies and serum anti-LC3 (recombinant cysteine-rich lectin protein) IgG antibodies were positive predictors for E. histolytica infection (P < 0.03). Of interest, infection with Trichomonas hominis but not Blastocystis hominis was positively associated with E. histolytica infection (P < 0.05). In conclusion, ELISA for detection of fecal lectin antigen is a more sensitive method than fecal culture for detecting asymptomatic E. histolytica infection.
我们在埃及米努夫省的卡费尔·达乌德村开展了一项前瞻性现场研究，比较粪便培养法与酶联免疫吸附测定（ELISA）检测粪便中170 kDa凝集素抗原以诊断无症状溶组织内阿米巴和迪斯帕内阿米巴感染的效果。通过培养检测出溶组织内阿米巴或迪斯帕内阿米巴感染的所有受试者，其粪便中的阿米巴抗原ELISA检测结果也呈阳性，并且ELISA还检测出另外57例培养漏检的内阿米巴感染（与培养法相比，P < 0.001）。粪便抗凝集素IgA抗体和血清抗LC3（重组富含半胱氨酸的凝集素蛋白）IgG抗体的存在是溶组织内阿米巴感染的阳性预测指标（P < 0.03）。有趣的是，人毛滴虫感染而非人芽囊原虫感染与溶组织内阿米巴感染呈正相关（P < 0.05）。总之，检测粪便凝集素抗原的ELISA法在检测无症状溶组织内阿米巴感染方面比粪便培养法更敏感。
