Al-Hindi Adnan, Shubair Mohammad E, Marshall Ian, Ashford Ricahrd W, Sharif Fadel A, Abed Abdalla A, Kamel Eryan G
The Islamic University of Gaza, Faculty of Science, Depart -ments of Biology and Medical Technology, PO Box 108, Gaza, Palestine National Authority. ahindi@mail iugaza
J Egypt Soc Parasitol. 2005 Apr;35(1):59-68.
Most physicians in Gaza prescribe medicaments for patient's suffering from Entamoeba histolytica/dispar without parasitologic diagnosis. Additionally, stool analysis performed by the routine methods usually reports the species as E. histolytica without con-firmation. In this study, 92 stool specimens were collected and analyzed by wet mount, iron haematoxylin staining, antigen detection of E. histolytica and polymerase chain reaction (PCR). The total number of E. histolytica identified by PCR was 64 (69.6%) that of E. dispar was 21 (22.8%). Mixed infection with both E. histolytica and E. dispar was evident in 7 specimens (7.6%). In the light of these results approximately 30% of suspected clinical amoebiasis cases were negative for E. histolytica. It is recommended to use PCR for diagnosis of stool specimens from patients with E. histolytica/dispar and that treatment should be prescribed for only patients positive for E.
加沙地带的大多数医生在未进行寄生虫学诊断的情况下就为患有溶组织内阿米巴/迪斯帕内阿米巴感染的患者开药方。此外，采用常规方法进行的粪便分析通常在未经确认的情况下就将该物种报告为溶组织内阿米巴。在本研究中，收集了92份粪便标本，并通过湿片法、铁苏木精染色、溶组织内阿米巴抗原检测和聚合酶链反应（PCR）进行分析。通过PCR鉴定出的溶组织内阿米巴总数为64例（69.6%），迪斯帕内阿米巴为21例（22.8%）。在7份标本（7.6%）中明显存在溶组织内阿米巴和迪斯帕内阿米巴的混合感染。根据这些结果，大约30%疑似临床阿米巴病病例的溶组织内阿米巴检测呈阴性。建议对患有溶组织内阿米巴/迪斯帕内阿米巴感染的患者的粪便标本采用PCR进行诊断，并且仅应对溶组织内阿米巴检测呈阳性的患者开药治疗。
