Ma K, Roy A M, Whittaker G R
Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853, USA.
Virology. 2001 Apr 10;282(2):215-20. doi: 10.1006/viro.2001.0833.
A critical phase of the influenza virus life cycle is the regulated translocation of genomic ribonucleoproteins (vRNPs) from the nuclear interior, across the nuclear envelope, and into the cytoplasm. Two viral proteins, M1 and NS2, have previously been implicated as mediators of vRNP export. We show here that vRNP nuclear export is prevented by leptomycin B (LMB), an inhibitor of the cellular factor CRM1. In LMB-treated cells, vRNPs were found in a peripheral nuclear location that localized with the nuclear lamina. vRNPs were not colocalized with either M1 or NS2. In situ extraction of cells late in infection also revealed a peripheral localization of nuclear vRNPs, whereas early in infection vRNPs were dispersed throughout the nuclear interior. We believe that vRNPs at the nuclear periphery represent a novel intermediate in the influenza virus nuclear export pathway.
流感病毒生命周期的一个关键阶段是基因组核糖核蛋白（vRNP）从细胞核内部经核膜有调控地转运至细胞质。此前已表明两种病毒蛋白M1和NS2是vRNP输出的介质。我们在此表明，细胞因子CRM1的抑制剂雷帕霉素B（LMB）可阻止vRNP的核输出。在经LMB处理的细胞中，vRNP定位于细胞核周边且与核纤层共定位。vRNP与M1或NS2均未共定位。感染后期细胞的原位抽提也显示核vRNP定位于细胞核周边，而感染早期vRNP分散于整个细胞核内部。我们认为细胞核周边的vRNP代表了流感病毒核输出途径中的一种新型中间体。
