酿酒酵母大小控制基因WHI3的分离与鉴定
Isolation and characterization of WHI3, a size-control gene of Saccharomyces cerevisiae.
作者信息
Nash R S, Volpe T, Futcher B
机构信息
Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794-5222.
出版信息
Genetics. 2001 Apr;157(4):1469-80. doi: 10.1093/genetics/157.4.1469.
Abstract
WHI3 is a gene affecting size control and cell cycle in the yeast Saccharomyces cerevisiae. The whi3 mutant has small cells, while extra doses of WHI3 produce large cells, and a large excess of WHI3 produces a lethal arrest in G1 phase. WHI3 seems to be a dose-dependent inhibitor of Start. Whi3 and its partially redundant homolog Whi4 have an RNA-binding domain, and mutagenesis experiments indicate that this RNA-binding domain is essential for Whi3 function. CLN3-1 whi3 cells are extremely small, nearly sterile, and largely nonresponsive to mating factor. Fertility is restored by deletion of CLN2, suggesting that whi3 cells may have abnormally high levels of CLN2 function.
摘要
WHI3是一种影响酿酒酵母大小控制和细胞周期的基因。whi3突变体具有小细胞，而额外剂量的WHI3会产生大细胞，大量过量的WHI3会导致G1期致死性停滞。WHI3似乎是Start的剂量依赖性抑制剂。Whi3及其部分冗余的同源物Whi4具有一个RNA结合结构域，诱变实验表明该RNA结合结构域对Whi3的功能至关重要。CLN3-1 whi3细胞极其微小，几乎不育，并且对交配因子基本无反应。通过缺失CLN2可恢复育性，这表明whi3细胞可能具有异常高水平的CLN2功能。
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本文引用的文献
1
Autoregulated expression of Schizosaccharomyces pombe meiosis-specific transcription factor Mei4 and a genome-wide search for its target genes.粟酒裂殖酵母减数分裂特异性转录因子Mei4的自动调控表达及其全基因组靶基因搜索。
Genetics. 2000 Apr;154(4):1497-508. doi: 10.1093/genetics/154.4.1497.
2
The yeast proteome database (YPD) and Caenorhabditis elegans proteome database (WormPD): comprehensive resources for the organization and comparison of model organism protein information.酵母蛋白质组数据库（YPD）和秀丽隐杆线虫蛋白质组数据库（WormPD）：用于组织和比较模式生物蛋白质信息的综合资源。
Nucleic Acids Res. 2000 Jan 1;28(1):73-6. doi: 10.1093/nar/28.1.73.
3
Saccharomyces cerevisiae G1 cyclins are differentially involved in invasive and pseudohyphal growth independent of the filamentation mitogen-activated protein kinase pathway.酿酒酵母G1细胞周期蛋白以独立于丝状化丝裂原活化蛋白激酶途径的方式，不同程度地参与侵袭性生长和假菌丝生长。
Genetics. 1999 Dec;153(4):1535-46. doi: 10.1093/genetics/153.4.1535.
4
Connections between growth and the cell cycle.生长与细胞周期之间的联系。
Curr Opin Cell Biol. 1998 Dec;10(6):784-90. doi: 10.1016/s0955-0674(98)80122-1.
5
Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization.通过微阵列杂交全面鉴定酿酒酵母细胞周期调控基因。
Mol Biol Cell. 1998 Dec;9(12):3273-97. doi: 10.1091/mbc.9.12.3273.
6
The transcriptional program of sporulation in budding yeast.芽殖酵母中孢子形成的转录程序。
Science. 1998 Oct 23;282(5389):699-705. doi: 10.1126/science.282.5389.699.
7
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8
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Mol Cell Biol. 1998 Jul;18(7):3681-91. doi: 10.1128/MCB.18.7.3681.
9
Coupling of cell division to cell growth by translational control of the G1 cyclin CLN3 in yeast.通过酵母中G1细胞周期蛋白CLN3的翻译控制实现细胞分裂与细胞生长的偶联。
Genes Dev. 1997 Oct 1;11(19):2522-31. doi: 10.1101/gad.11.19.2522.
10
Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae.通过转座子诱变对酿酒酵母丝状生长进行剖析。
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