Chu S, DeRisi J, Eisen M, Mulholland J, Botstein D, Brown P O, Herskowitz I
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143-0448, USA.
Science. 1998 Oct 23;282(5389):699-705. doi: 10.1126/science.282.5389.699.
Diploid cells of budding yeast produce haploid cells through the developmental program of sporulation, which consists of meiosis and spore morphogenesis. DNA microarrays containing nearly every yeast gene were used to assay changes in gene expression during sporulation. At least seven distinct temporal patterns of induction were observed. The transcription factor Ndt80 appeared to be important for induction of a large group of genes at the end of meiotic prophase. Consensus sequences known or proposed to be responsible for temporal regulation could be identified solely from analysis of sequences of coordinately expressed genes. The temporal expression pattern provided clues to potential functions of hundreds of previously uncharacterized genes, some of which have vertebrate homologs that may function during gametogenesis.
芽殖酵母的二倍体细胞通过孢子形成的发育程序产生单倍体细胞，该程序包括减数分裂和孢子形态发生。使用包含几乎每个酵母基因的DNA微阵列来检测孢子形成过程中的基因表达变化。观察到至少七种不同的诱导时间模式。转录因子Ndt80似乎对减数分裂前期结束时一大组基因的诱导很重要。仅通过对协同表达基因的序列分析，就可以识别已知或推测负责时间调控的共有序列。时间表达模式为数百个以前未表征基因的潜在功能提供了线索，其中一些基因具有可能在配子发生过程中起作用的脊椎动物同源物。
