Ebeling D D, Westphall M S, Scalf M, Smith L M
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706, USA.
Rapid Commun Mass Spectrom. 2001;15(6):401-5. doi: 10.1002/rcm.245.
A cylindrical capacitor ionization source was used in conjunction with corona discharge charge reduction for generation of singly charged ions for mass spectrometric analysis. The source consists of a fused-silica capillary threaded with a platinum wire and placed inside a stainless steel tube. Application of an electric potential to the wire results in the production of a linear stream of charged droplets when an aqueous solution is pumped through the capillary. Subsequent solvent evaporation yields ions, providing a continuous ion source for mass spectrometry. Passage of the ions through a corona discharge charge reduction chamber permits reduction of the charge state to predominantly singly charged species, facilitating analysis of DNA and protein mixtures. The change from production of multiply charged ions to production of singly charged ions is extremely simple, requiring only modulation of the voltage applied to the corona discharge electrode. A simple technique for construction of the ionization source is reported.
一种圆柱形电容器电离源与电晕放电电荷减少技术结合使用，用于产生单电荷离子以进行质谱分析。该源由一根穿有铂丝的熔融石英毛细管组成，并放置在不锈钢管内。当水溶液通过毛细管泵送时，对金属丝施加电势会产生线性的带电液滴流。随后的溶剂蒸发产生离子，为质谱分析提供了连续的离子源。离子通过电晕放电电荷减少室可使电荷态主要降低为单电荷物种，便于对DNA和蛋白质混合物进行分析。从产生多电荷离子转变为产生单电荷离子极其简单，仅需调节施加到电晕放电电极的电压。本文报道了一种构建电离源的简单技术。
