Stenmark H, Gillooly D J
Department of Biochemistry, the Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway.
Semin Cell Dev Biol. 2001 Apr;12(2):193-9. doi: 10.1006/scdb.2000.0236.
Phosphatidylinositol 3-kinases (PI 3-kinases) regulate cellular functions through the 3'-phosphorylation of phosphatidylinositol (PI) and its derivatives. The PI 3-kinase product phosphatidylinositol 3-phosphate [PI(3)P] functions to recruit and activate effector proteins containing FYVE zinc finger domains. These proteins have various functions in endocytic membrane trafficking, cytoskeletal regulation and signal transduction. In order to understand the function of FYVE proteins, it is essential to study the formation, localisation, trafficking and turnover of PI(3)P. Here we review recent evidence that PI(3)P is formed on early endosomes through the activity of a PI 3-kinase which is recruited by the GTPase Rab5, and that the PI(3)P is subsequently internalised into intralumenal vesicles of multivesicular endosomes for turnover.
磷脂酰肌醇3激酶（PI 3激酶）通过磷脂酰肌醇（PI）及其衍生物的3'-磷酸化来调节细胞功能。PI 3激酶产物磷脂酰肌醇3-磷酸[PI(3)P]的作用是募集并激活含有FYVE锌指结构域的效应蛋白。这些蛋白在内吞膜运输、细胞骨架调节和信号转导中具有多种功能。为了了解FYVE蛋白的功能，研究PI(3)P的形成、定位、运输和周转至关重要。在此，我们综述了近期的证据，即PI(3)P通过GTP酶Rab5募集的PI 3激酶的活性在早期内体上形成，随后PI(3)P被内化到多泡内体的腔内小泡中进行周转。
