Booth C W, Nader H L
Proc Soc Exp Biol Med. 1975 Jan;148(1):277-82. doi: 10.3181/00379727-148-38521.
The accumulation of neutral amino acids was studied in cultivated human skin fibroblasts. Transport was blocked by sulfhydryl binding agents, but proceeded without inhibition in the presence of metabolic blocking agents. Both sodium dependent and sodium independent compononets of neutral amino acid accumulation were found. The sodium dependent component preferentially transports short chain amino acids, while the sodium independent system transports long chain amino acids. These components operate simultaneously and with overlap of substrate specificity. Both may function as parts of a single neutral amino acid transport system.
在培养的人皮肤成纤维细胞中研究了中性氨基酸的积累。转运被巯基结合剂阻断，但在代谢阻断剂存在的情况下仍能继续进行且不受抑制。发现中性氨基酸积累既有钠依赖性成分，也有钠非依赖性成分。钠依赖性成分优先转运短链氨基酸，而钠非依赖性系统转运长链氨基酸。这些成分同时起作用且底物特异性有重叠。两者都可能作为单一中性氨基酸转运系统的组成部分发挥作用。
