用于DNA的新型分子“光开关”的设计
The design of new molecular "light switches" for DNA.
作者信息
Zou X H, Ye B H, Li H, Zhang Q L, Chao H, Liu J G, Ji L N, Li X Y
机构信息
Department of Chemistry, Zhongshan University, Guangzhou, PR China.
出版信息
J Biol Inorg Chem. 2001 Feb;6(2):143-50. doi: 10.1007/s007750000183.
Two novel ruthenium(II) complexes, Ru(pztp)2(phen)2 and [Ru(pztp)2(bpy)] (ClO4)2, have been synthesized and characterized by UV/Vis and 1H NMR spectroscopies and mass spectrometry. The MeCN solutions of both complexes display fluorescence that was found to be highly sensitive to the presence and concentration of water. The complexes behave like a "light switch" for DNA in that they do not luminesce in water but were "turned on" in the presence of DNA and show emission enhancement with the increase of DNA concentration. Their DNA binding behavior was also studied by absorption spectroscopy and viscosity measurements, which suggest that the DNA-complex interaction involves intercalation of the metal-bound pztp ligand into the base pairs of duplex DNA.
合成了两种新型钌（II）配合物，Ru(pztp)2(phen)2 和 [Ru(pztp)2(bpy)] (ClO4)2，并通过紫外可见光谱、1H核磁共振光谱和质谱对其进行了表征。两种配合物的乙腈溶液均显示出荧光，发现该荧光对水的存在和浓度高度敏感。这些配合物对DNA的作用类似于“光开关”，即在水中不发光，但在DNA存在时“开启”，并随着DNA浓度的增加而显示出发射增强。还通过吸收光谱和粘度测量研究了它们与DNA的结合行为，结果表明DNA与配合物的相互作用涉及金属结合的pztp配体插入双链DNA的碱基对中。
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