Domino E F, Wilson A E, Olds M E
Pharmacol Biochem Behav. 1975 Jan-Feb;3(1):103-7. doi: 10.1016/0091-3057(75)90087-8.
Total brain acetylcholine (ACh) was assayed in groups of animals after various periods of operant responding maintained by electrical stimulation of the lateral posterior hypothalamus or of escape behavior induced by electrical stimulation of the midbrain tegmentum. Different groups of trained rats were placed in identical Skinner boxes for periods of 1 to 24 hr. The following groups were studied: controls, self-stimulators receiving electrical stimulation, escapers from brain stimulation or peripherally applied aversive stimulation, self-stimulators not receiving electrical stimulation prior to decapitation, tubocurarine-paralyzed respired rats with electrodes in the posterior-lateral hypothalamus not receiving stimulation, and a group of tubocurarine-paralyzed, respired rats receiving electrical stimulation automatically. It was found that brain stimulation decreased total brain ACh, regardless of whether the stimulation was positive, as during self-stimulation behavior, or negative, as during escape behavior. Animals that receivied positive stimulation while being paralyzed showed similar decreases in total brain ACh, but the change in ACh was smaller. No changes occurred in animals that were paralyzed that recieved no electrical stimulation. It is concluded that brain usage produced by electrical stimulation of discrete functional pathways causes a reduction of total ACh, but this is unrelated to the specific motivational properties of the electrical stimuli.
在通过电刺激下丘脑后侧或中脑被盖诱发的逃避行为维持的不同时期操作性反应后，对几组动物的全脑乙酰胆碱（ACh）进行了测定。将不同组的训练大鼠置于相同的斯金纳箱中1至24小时。研究了以下几组：对照组、接受电刺激的自我刺激者、逃避脑刺激或外周施加厌恶刺激者、断头前未接受电刺激的自我刺激者、在后下丘脑外侧植入电极但未接受刺激的筒箭毒碱麻痹的呼吸大鼠，以及一组自动接受电刺激的筒箭毒碱麻痹的呼吸大鼠。结果发现，无论刺激是积极的（如自我刺激行为期间）还是消极的（如逃避行为期间），脑刺激都会降低全脑ACh。在麻痹状态下接受积极刺激的动物全脑ACh也有类似的降低，但ACh的变化较小。未接受电刺激的麻痹动物没有变化。得出的结论是，电刺激离散功能通路产生的脑活动导致全ACh减少，但这与电刺激的特定动机特性无关。
