Mileva M, Zlateva G, Karabasheva S, Hadjimitova V, Antonov I
Department of Physics and Biophysics, Medical University, Sofia, Bulgaria.
Methods Find Exp Clin Pharmacol. 2000 Nov;22(9):679-81. doi: 10.1358/mf.2000.22.9.802283.
The effect of low-intensity laser irradiation on the processes of lipid peroxidation in lens homogenate and aqueous humor during experimental diquat-induced cataract of rabbit eyes was studied. The levels of primary and secondary products of lipid peroxidation (LPO), conjugated dienes and thiobarbituric acid reactive substances (TBARS), were evaluated. We found that the experimental cataract model leads to a significant increase in the content of conjugated dienes and in the content of TBARS both in lens homogenate and in aqueous humor. The data obtained support the important role of oxidative stress in the development of the diquat-induced cataract model. Low-intensity laser treatment does not provoke a significant decrease in conjugated dienes or in TBARS in either lens homogenate or aqueous humor. Although our therapeutic scheme led to a slightly decreased level of LPO products, we conclude that the effect of low-intensity laser-irradiation may depend on the dose applied, individual tissues and other factors.
研究了低强度激光照射对兔眼实验性敌草快诱导白内障过程中晶状体匀浆和房水中脂质过氧化过程的影响。评估了脂质过氧化（LPO）的初级和次级产物、共轭二烯和硫代巴比妥酸反应性物质（TBARS）的水平。我们发现，实验性白内障模型导致晶状体匀浆和房水中共轭二烯含量和TBARS含量显著增加。获得的数据支持氧化应激在敌草快诱导的白内障模型发展中的重要作用。低强度激光治疗在晶状体匀浆或房水中均未引起共轭二烯或TBARS的显著降低。尽管我们的治疗方案导致LPO产物水平略有下降，但我们得出结论，低强度激光照射的效果可能取决于所应用的剂量、个体组织和其他因素。
