Li Y, Chen J, Chopp M
Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI 48202, USA.
Cell Transplant. 2001 Jan-Feb;10(1):31-40.
We transplanted adult whole bone marrow prelabeled with bromodeoxyuridine (BrdU) into the ischemic boundary zone of the adult rat brain at 1 day after 2 h of middle cerebral artery occlusion (MCAo). Approximately 3.3% of 10(6) transplanted bone marrow cells were BrdU reactive at 14 days after MCAo. BrdU-reactive cells expressed neuronal and astrocytic proteins, neuronal nuclei protein (NeuN, 1%), and glial fibrillary acidic protein (GFAP, 5%) immunoreactivities, respectively. In addition, bone marrow transplantation promoted proliferation of ependymal and subependymal cells, identified by nestin (a neuroepithelial stem cell marker), within the ventricular zone and subventricular zone (VZ/SVZ). These data suggest that intracerebral transplantation of bone marrow could potentially be used to induce plasticity in ischemic brain.
在大脑中动脉闭塞（MCAo）2小时后1天，我们将预先用溴脱氧尿苷（BrdU）标记的成年大鼠全骨髓移植到成年大鼠脑缺血边界区。在MCAo后14天，约3.3%的10(6)个移植骨髓细胞呈BrdU反应性。BrdU反应性细胞分别表达神经元和星形胶质细胞蛋白、神经元核蛋白（NeuN，1%）和胶质纤维酸性蛋白（GFAP，5%）免疫反应性。此外，骨髓移植促进了脑室区和室下区（VZ/SVZ）中由巢蛋白（一种神经上皮干细胞标志物）鉴定的室管膜和室管膜下细胞的增殖。这些数据表明，骨髓脑内移植可能潜在地用于诱导缺血性脑的可塑性。
