Kim Jung Yeon, Chun So Young, Park Jin-Sung, Chung Jae-Wook, Ha Yun-Sok, Lee Jun Nyung, Kwon Tae Gyun
1Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, 135 Dongdeok-ro, Jung-gu, Daegu, 41940 Korea.
2Department of Neurology, School of Medicine, Kyungpook National University, 807 Hoguk-ro, Buk-gu, Daegu, 41404 Korea.
Tissue Eng Regen Med. 2017 Dec 28;15(2):195-209. doi: 10.1007/s13770-017-0102-x. eCollection 2018 Apr.
Urine-derived stem cells (USCs) are considered as a promising cell source capable of neuronal differentiation. In addition, specific growth factors and extracellular matrix are essential for enhancing their neuronal differentiation efficiency. In this study, we investigated the possibility of neuronal differentiation of USCs and the role of laminin and platelet-derived growth factor BB (PDGF-BB) as promoting factors. USCs were isolated from fresh urine of healthy donors. Cultured USCs were adherent to the plate and their morphology was similar to the cobblestone. In addition, they showed chromosome stability, rapid proliferation rate, colony forming capacity, and mesenchymal stem cell characteristics. For inducing the neuronal differentiation, USCs were cultured for 14 days in neuronal differentiation media supplemented with/without laminin and/or PDGF-BB. To identify the expression of neuronal markers, RT-PCR, flow cytometry analysis and immunocytochemistry were used. After neuronal induction, the cells showed neuron-like morphological change and high expression level of neuronal markers. In addition, laminin and PDGF-BB respectively promoted the neuronal differentiation of USCs and the combination of laminin and PDGF-BB showed a synergistic effect for the neuronal differentiation of USCs. In conclusion, USCs are noteworthy cell source in the field of neuronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency.
尿液来源的干细胞（USCs）被认为是一种有前景的能够进行神经元分化的细胞来源。此外，特定的生长因子和细胞外基质对于提高其神经元分化效率至关重要。在本研究中，我们研究了USCs神经元分化的可能性以及层粘连蛋白和血小板衍生生长因子BB（PDGF-BB）作为促进因子的作用。USCs从健康供体的新鲜尿液中分离得到。培养的USCs贴壁生长，其形态类似于鹅卵石。此外，它们表现出染色体稳定性、快速增殖率、集落形成能力以及间充质干细胞特征。为了诱导神经元分化，将USCs在添加/不添加层粘连蛋白和/或PDGF-BB的神经元分化培养基中培养14天。使用RT-PCR、流式细胞术分析和免疫细胞化学来鉴定神经元标志物的表达。神经元诱导后，细胞呈现出神经元样形态变化以及神经元标志物的高表达水平。此外，层粘连蛋白和PDGF-BB分别促进了USCs的神经元分化，层粘连蛋白和PDGF-BB的组合对USCs的神经元分化表现出协同作用。总之，USCs是神经元再生领域值得关注的细胞来源，层粘连蛋白和PDGF-BB提高了它们的神经元分化效率。
