Abnormal expression of pan-ras, c-myc and tp53 in squamous cell carcinoma of cervix: correlation with HPV and prognosis.

The aim of this study is to assess, in squamous cell carcinoma of the cervix, the expression of pan-ras, c-myc and tp53 at protein level using an immunohistochemical (IHC) staining method. One hundred and seven patients with squamous cell carcinoma of the cervix were recruited. Fifty-four patients were of stage 1B/2A and 53 were of stage 2B and above. Positive IHC stainings of pan-ras, c-myc and tp53 proteins were detected in 80.4%, 32.7% and 25.2% of cases, respectively. No significant correlation between overexpression of pan-ras and c-myc was detected. However, significantly higher percentages of overexpression of c-myc was found in association with overly expressed tp53 samples (p = 0.014). Human papillomavirus (HPV) was detected in 77.6% of cancers. HPV 16/18 was detected in 72% of cancers. Overexpression of pan-ras and c-myc had no correlation with HPV detection and stage. However, higher percentages of overexpression of tp53 were found in early stage disease (p = 0.017) and in HPV 16/18 positive tumors (p = 0.006). Overexpression of pan-ras, c-myc and tp53 alone or in more than one oncogenes had no prognostic significance on survival.