Sanchez Lockhart M, Cabrera P, Diament M, Alvarez E, Klein D, Hajos S E
Cátedra de Inmunología, IDEHU-CONICET, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Int J Mol Med. 2001 May;7(5):557-62. doi: 10.3892/ijmm.7.5.557.
CD44 is a widely distributed set of cell surface glycoproteins expressed in several types of cells and tissues, implicated in cell-cell and cell-substrate interactions. This molecule plays a major role in cell differentiation, development and activation and has also been described as a potential marker of malignancy and metastasis. In the present study we investigated by RT-PCR followed by exon specific amplification the expression of CD44 splice variants in four different murine tumors as well as in the invaded organs in order to correlate the expression of CD44 variants with potential tumor invasiveness and their implications for growth. Our data showed deregulation in the expression of CD44 isoforms but no discernible correlation in isoform expression pattern. However, in all tumors studied isoforms presented by the primary tumor were detected in the invaded organs before metastasis could be demonstrated by histopathological analysis.
CD44是一组广泛分布的细胞表面糖蛋白，在多种类型的细胞和组织中表达，参与细胞间和细胞与基质的相互作用。该分子在细胞分化、发育和激活中起主要作用，也被描述为恶性肿瘤和转移的潜在标志物。在本研究中，我们通过逆转录聚合酶链反应（RT-PCR）继以外显子特异性扩增，研究了四种不同小鼠肿瘤以及侵袭器官中CD44剪接变体的表达，以便将CD44变体的表达与潜在的肿瘤侵袭性及其对生长的影响相关联。我们的数据显示CD44异构体的表达失调，但异构体表达模式中没有明显的相关性。然而，在所有研究的肿瘤中，在通过组织病理学分析证实转移之前，原发肿瘤呈现的异构体已在侵袭器官中被检测到。
