体外研究3-[123I]碘-L-α-甲基酪氨酸（[123I]IMT）转运进入人尤因肉瘤细胞的特性。
Characterization of 3-[123I]iodo-L-alpha-methyl tyrosine ([123I]IMT) transport into human Ewing's sarcoma cells in vitro.
作者信息
Franzius C, Kopka K, van Valen F, Eckervogt V, Riemann B, Sciuk J, Schober O
机构信息
Department of Nuclear Medicine, Westfälische Wilhelms-Universität, Münster, Albert-Schweitzer-Str. 33, 48149, Münster, Germany.
出版信息
Nucl Med Biol. 2001 Feb;28(2):123-8. doi: 10.1016/s0969-8051(00)00186-4.
3-[(123)I]Iodo-L-alpha-methyl tyrosine ([(123)I]IMT) scintigraphy of extracranial malignant tumors has been described, but little is known about the transport systems involved in [(123)I]IMT uptake into extracranial tumor cells. Here, the precise kinetics of [(123)I]IMT transport into human Ewing's sarcoma cells (VH-64) was determined. The apparent Michaelis constant was of high affinity value (K(m)=41.7+/-3.9 microM) and maximum transport velocitiy amounted to V(max)=20.7+/-0.6 nmol x mg protein(-1) x 10 min(-1). Inhibition experiments revealed the predominance of [(123)I]IMT uptake via sodium-independent system L.
3-[(123)I]碘-L-α-甲基酪氨酸([(123)I]IMT)对颅外恶性肿瘤的闪烁扫描已见报道，但关于[(123)I]IMT摄入颅外肿瘤细胞所涉及的转运系统却知之甚少。在此，测定了[(123)I]IMT转运至人尤因肉瘤细胞(VH-64)的精确动力学。表观米氏常数具有高亲和力值(K(m)=41.7±3.9微摩尔)，最大转运速度达V(max)=20.7±0.6纳摩尔×毫克蛋白(-1)×10分钟(-1)。抑制实验显示[(123)I]IMT通过非钠依赖的L系统摄入占主导。
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