3-[123I]碘-L-α-甲基酪氨酸向人成纤维细胞的转运及其与尤因肉瘤细胞的比较。

3-[123I]Iodo-L-alpha-methyl tyrosine transport into human fibroblasts and comparison with Ewing's sarcoma cells.

作者信息

Franzius Christiane, Kopka Klaus, van Valen Frans, Riemann Burkhard, Sciuk Joachim, Schober Otmar

机构信息

Department of Nuclear Medicine, University Hospital, Muenster, Germany.

出版信息

Nucl Med Biol. 2002 May;29(4):483-90. doi: 10.1016/s0969-8051(02)00291-3.

DOI:10.1016/s0969-8051(02)00291-3

PMID:12031884

Abstract

The cellular transport systems and the transport kinetics of [123I]IMT uptake into non-malignant extracranial cells were characterized for the first time. Human fibroblasts were chosen as non-malignant extracranial cells as they are found ubiquitous in the body. [123I]IMT is exclusively transported into fibroblasts via the sodium independent system L. An apparent Michaelis constant K(m) = 116.2 +/- 18.9 microM and a maximum transport velocity V(max) = 191.6 +/- 13.9 pmol x (10(6) cells)(-1) x min(-1) were calculated for the sodium-independent transport. These results were compared with those determined in two malignantly transformed extracranial cell lines, the human Ewing's sarcoma cell lines VH-64 and CADO-ES-1.

摘要

首次对[123I]IMT摄取进入非恶性颅外细胞的细胞转运系统及转运动力学进行了表征。人成纤维细胞被选作非恶性颅外细胞，因为它们在人体中普遍存在。[123I]IMT仅通过非钠依赖系统L转运进入成纤维细胞。计算得出非钠依赖转运的表观米氏常数K(m)=116.2±18.9微摩尔，最大转运速度V(max)=191.6±13.9皮摩尔×(10^6个细胞)^(-1)×分钟^(-1)。将这些结果与在两种恶性转化的颅外细胞系，即人尤因肉瘤细胞系VH - 64和CADO - ES - 1中测定的结果进行了比较。