Karu T I, Pyatibrat L V, Kalendo G S
Institute of Laser and Informatic Technologies, Russian Academy of Sciences, 142190 Troitsk, Moscow Region, Russian Federation.
Lasers Surg Med. 2001;28(3):227-36. doi: 10.1002/lsm.1043.
Adhesive interactions between cells and extracellular matrices play a regulative role in wound repair processes. The objective of this investigation is to study the mechanisms of light action on cellular adhesion in vitro. The adhesion of HeLa cells to a glass matrix is evaluated after irradiation with a pulsed near-infrared (IR) diode and treatment with various chemicals.
STUDY DESIGN/MATERIALS AND METHODS: A semiconductor diode (820 +/- 10 nm, 10Hz, 16--120 J/m(2)) is used for irradiation of the cell suspension. In parallel experiments, various chemicals (mannitol, melatonin, ethanol, ascorbic acid, superoxide dismutase, catalase, rotenone, azide, dinitrophenol (DNP), methylene blue, and hydrogen peroxide) are added to the cell suspension before or after the irradiation procedure. The cell-glass adhesion is studied by using the adhesion assay technique (Lasers Surg. Med. 1996;18:171).
It has been found that cell-glass adhesion increases in a dose-dependent manner after irradiation. The treatment of the cells with antioxidants (free radical scavengers), e.g., mannitol, melatonin, ethanol, and ascorbic acid, as well as with the ionophore DNP, eliminated the light effect. The respiratory chain inhibitors rotenone and azide strongly modified the light effect, depending on the dose. The oxidative agents hydrogen peroxide (in a low concentration) and methylene blue increased the cell adhesion. Superoxide dismutase did not modify the light effect. The effect of the catalase (stimulative or suppressive) was dependent on its concentration and treatment sequence. Preirradiation was found to decrease (or normalize to the control level) the suppressive effects of some chemicals.
The results obtained are evidence that first, pulsed IR radiation with certain parameters modulates the cell-matrix attachment. second, free radical and redox processes are involved in the cell-matrix interaction, probably at some stage(s) of the photosignal transduction. Third, both types of the primary reactions in the respiratory chain, namely, the increase of the electron flow and production of the reactive oxygen species, cause a transient oxidative stress in the cytoplasm.
细胞与细胞外基质之间的黏附相互作用在伤口修复过程中起调节作用。本研究的目的是探讨光作用于体外细胞黏附的机制。在用脉冲近红外（IR）二极管照射并用各种化学物质处理后，评估HeLa细胞与玻璃基质的黏附情况。
研究设计/材料与方法：使用半导体二极管（820±10nm，10Hz，16 - 120J/m²）照射细胞悬液。在平行实验中，在照射程序之前或之后，将各种化学物质（甘露醇、褪黑素、乙醇、抗坏血酸、超氧化物歧化酶、过氧化氢酶、鱼藤酮、叠氮化物、二硝基苯酚（DNP）、亚甲蓝和过氧化氢）添加到细胞悬液中。通过使用黏附测定技术（《激光外科与医学》1996年；18:171）研究细胞与玻璃的黏附情况。
已发现照射后细胞与玻璃的黏附以剂量依赖性方式增加。用抗氧化剂（自由基清除剂），如甘露醇、褪黑素、乙醇和抗坏血酸，以及离子载体DNP处理细胞，消除了光效应。呼吸链抑制剂鱼藤酮和叠氮化物根据剂量强烈改变了光效应。氧化剂过氧化氢（低浓度）和亚甲蓝增加了细胞黏附。超氧化物歧化酶未改变光效应。过氧化氢酶的作用（刺激或抑制）取决于其浓度和处理顺序。发现预照射可降低（或使其恢复到对照水平）某些化学物质的抑制作用。
获得的结果证明，首先，具有特定参数的脉冲红外辐射调节细胞与基质的附着。其次，自由基和氧化还原过程参与细胞与基质的相互作用，可能在光信号转导的某些阶段。第三，呼吸链中的两种类型的初级反应，即电子流的增加和活性氧的产生，在细胞质中引起短暂的氧化应激。
