Karu T I, Pyatibrat L V, Kalendo G S
Institute of Laser and Informatic Technologies, Russian Academy of Sciences, 142190 Troitsk, Moscow, Russia.
Lasers Surg Med. 2001;29(3):274-81. doi: 10.1002/lsm.1119.
Adhesive interactions between cells and extracellular matrices play a regulative role in wound repair processes. The objective of this investigation is to study action mechanisms of pulsed radiation at 820 nm on cellular adhesion in vitro. Light emitting diodes (LED) at 820 nm are widely used for treatment of wounds of various etiology.
STUDY DESIGN/MATERIALS AND METHODS: The LED (820 +/- 10 nm, 10 Hz, 16-120 J/m(2)) is used for the irradiation of HeLa cell suspension. In parallel experiments, amiloride (5 x 10(-4) M), ouabain (7 x 10(-5) M, 7 x 10(-4) M), quinacrine (6 x 10(-4) M), arachidonic acid (1 x 10(-5) M), glucose (2 x 10(-4) M), and ATP (5 x 10(-5) M) are added to the cell suspension before or after the irradiation procedure. The cell-glass adhesion is studied using the adhesion assay technique described in Lasers Surg Med 1996; 18:171.
Cell-glass adhesion increases in a dose-dependent manner following the irradiation. Preirradiation eliminates the inhibition of cell attachment caused by ouabain, arachidonic acid, and ATP. The inhibitive effect of quinacrine on the cell attachment is eliminated by the irradiation performed after the treatment with the chemical. Irradiation and amiloride have a synergetic stimulative effect on the cell attachment. The threshold dose for the cell attachment stimulation by the irradiation is decreased by the treatment of the cell suspension with amiloride or ouabain.
The results obtained indicate that pulsed IR radiation at 820 nm increases the cell-matrix attachment. It is the modulation of the monovalent ion fluxes through the plasma membrane and not the release of arachidonic acid that is involved in the cellular signaling pathways activated by irradiation at 820 nm. Preirradiation has a protective effect against the inhibitive action of ouabain, arachidonic acid, ATP, and quinacrine on cell attachment process. It is supposed that irradiation activates those signaling pathways in cells which attenuate the inhibitive action of these chemicals.
细胞与细胞外基质之间的黏附相互作用在伤口修复过程中发挥调节作用。本研究的目的是探讨820nm脉冲辐射对体外细胞黏附的作用机制。820nm发光二极管（LED）广泛用于治疗各种病因的伤口。
研究设计/材料与方法：用LED（820±10nm，10Hz，16 - 120J/m²）照射HeLa细胞悬液。在平行实验中，在照射程序之前或之后，将amiloride（5×10⁻⁴M）、哇巴因（7×10⁻⁵M、7×10⁻⁴M）、喹吖因（6×10⁻⁴M）、花生四烯酸（1×10⁻⁵M）、葡萄糖（2×10⁻⁴M）和ATP（5×10⁻⁵M）添加到细胞悬液中。使用《激光外科与医学》1996年第18卷第171页所述的黏附测定技术研究细胞与玻璃的黏附。
照射后细胞与玻璃的黏附呈剂量依赖性增加。预照射消除了哇巴因、花生四烯酸和ATP对细胞附着的抑制作用。喹吖因对细胞附着的抑制作用在用该化学物质处理后进行的照射消除。照射和amiloride对细胞附着有协同刺激作用。用amiloride或哇巴因处理细胞悬液可降低照射刺激细胞附着的阈值剂量。
所得结果表明，820nm脉冲红外辐射增加了细胞与基质的附着。参与820nm照射激活的细胞信号通路的是通过质膜的单价离子通量的调节，而不是花生四烯酸的释放。预照射对哇巴因、花生四烯酸、ATP和喹吖因对细胞附着过程的抑制作用具有保护作用。据推测，照射激活了细胞中的那些信号通路，这些信号通路减弱了这些化学物质的抑制作用。
