Teplitz N A
Sov J Dev Biol. 1975 Jul;5(4):344-9.
The incorporation of labeled amino acids into the total proteins of muscle tissue and cell organelles during the regeneration of limbs of axolotls and after the suppression of the regenerative capacity by x-irradiation and its experimental restoration were investigated. In the case of suppression of the regenerative capacity of the limbs by x-irradiation, the protein synthesis in the muscle tissue is impaired. The intensity of the incorporation of labeled amino acids into the proteins of a homogenate of muscle tissue, nuclei, mitochondria, and the mitochondria-free fraction is reduced in comparison with normal regeneration. Under the influence of biopreparations (a salt extract from a homogenate of the rat gastrocnemius muscle), a restoration of protein synthesis, processes of differentiation, and the formation of regenerates was observed. C-14-lysine was incorporated most intensively into the proteins of the mitochondria-free fraction and nuclei at the spatula stage (exceeding this index in the series with irradiation of the limbs without treatment with the biopreparation by 5 and 7-fold, respectively).
研究了在蝾螈肢体再生过程中以及通过X射线照射抑制再生能力并进行实验性恢复后，标记氨基酸掺入肌肉组织和细胞器总蛋白中的情况。在用X射线照射抑制肢体再生能力的情况下，肌肉组织中的蛋白质合成会受到损害。与正常再生相比，标记氨基酸掺入肌肉组织匀浆、细胞核、线粒体和无线粒体部分蛋白质中的强度降低。在生物制剂（大鼠腓肠肌匀浆的盐提取物）的影响下，观察到蛋白质合成、分化过程和再生组织形成的恢复。在匙状期，C-14-赖氨酸最强烈地掺入无线粒体部分和细胞核的蛋白质中（分别比未经生物制剂处理而照射肢体的系列中的该指标高出5倍和7倍）。
