Dees J H, Gazouli M, Papadopoulos V
Division of Hormone Research, Department of of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA.
Reprod Toxicol. 2001 Mar-Apr;15(2):171-87. doi: 10.1016/s0890-6238(01)00110-1.
We examined the effect of mono-ethylhexyl phthalate (MEHP) on MA-10 Leydig tumor cell structure and function. Cells were exposed to various concentrations of MEHP for 24 h and then stimulated with saturating concentrations of hCG for 2.5 h. Progesterone production, cell viability, and protein content were moderately inhibited by low concentrations and severely inhibited by high concentrations of MEHP. Electron microscopy showed a variety of alterations in the MEHP-treated cells, increasing in severity with increasing concentrations of MEHP. Lipid droplets were profoundly affected in the cells treated with MEHP and morphologic evidence that metabolism of lipid storage droplets ceases at approximately the same time progesterone synthesis stops was seen. Morphometric studies indicated that the number of lipid droplets appeared to be increased 2.5-fold over control levels at MEHP concentrations of 10(-6) to 10(-3) M whereas mitochondrial volume fraction decreased. These results suggest that MEHP in Leydig cells may act as a mitochondrial toxicant and lipid metabolism disrupter.
我们研究了邻苯二甲酸单-2-乙基己酯（MEHP）对MA-10睾丸间质细胞瘤细胞结构和功能的影响。将细胞暴露于不同浓度的MEHP中24小时，然后用饱和浓度的人绒毛膜促性腺激素（hCG）刺激2.5小时。低浓度的MEHP对孕酮生成、细胞活力和蛋白质含量有中度抑制作用，高浓度则有严重抑制作用。电子显微镜显示，经MEHP处理的细胞有多种改变，且随着MEHP浓度的增加，改变的严重程度也增加。在用MEHP处理的细胞中，脂滴受到严重影响，并且有形态学证据表明，脂质储存滴的代谢大约在孕酮合成停止的同时停止。形态计量学研究表明，在MEHP浓度为10^(-6)至10^(-3) M时，脂滴数量似乎比对照水平增加了2.5倍，而线粒体体积分数则下降。这些结果表明，睾丸间质细胞中的MEHP可能作为一种线粒体毒物和脂质代谢破坏剂发挥作用。
