Hillisch A, Lorenz M, Diekmann S
EnTec GmbH, Adolf-Reichwein-Strasse 20, D-07745 Jena, Germany.
Curr Opin Struct Biol. 2001 Apr;11(2):201-7. doi: 10.1016/s0959-440x(00)00190-1.
Fluorescence resonance energy transfer (FRET) provides information on the distance between a donor and an acceptor dye in the range 10 to 100 A. Knowledge of the exact positions of some dyes with respect to nucleic acids now enables us to translate these data into precise structural information using molecular modeling. Advances in the preparation of dye-labeled nucleic acid molecules and in new techniques, such as the measurement of FRET in polyacrylamide gels or in vivo, will lead to an increasingly important role of FRET in structural and molecular biology.
荧光共振能量转移（FRET）可提供供体染料与受体染料之间距离在10至100埃范围内的信息。目前，了解某些染料相对于核酸的确切位置，使我们能够利用分子建模将这些数据转化为精确的结构信息。染料标记核酸分子制备技术以及新技术（如在聚丙烯酰胺凝胶或体内测量FRET）的进展，将使FRET在结构生物学和分子生物学中发挥越来越重要的作用。
