肠道脂肪酸结合蛋白2的A54T多态性与糖耐量正常的白种人的胰岛素抵抗相关。

The A54T polymorphism at the intestinal fatty acid binding protein 2 is associated with insulin resistance in glucose tolerant Caucasians.

作者信息

Chiu K C, Chuang L M, Yoon C

机构信息

Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, University of California, Los Angeles, School of Medicine, Los Angeles, California, USA.

出版信息

BMC Genet. 2001;2:7. doi: 10.1186/1471-2156-2-7. Epub 2001 Mar 28.

DOI:10.1186/1471-2156-2-7

PMID:11299043

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC31346/

Abstract

BACKGROUND

An A54T polymorphism at the fatty acid binding protein 2 (FABP2) locus was found to be associated with insulin resistance in non-diabetic Pima Indians. To see whether this association is present in other populations, we performed a cross sectional study to examine the role of this polymorphism on insulin resistance in 55 healthy and normotensive Caucasian subjects with normal glucose tolerance. Insulin sensitivity (%S) and beta cell function (%B) were assessed using the Homeostasis Model Assessment (HOMA). Their genotypes were determined using a polymerase chain reaction-restriction fragment length polymorphism assay. The relationship between the genotypes and the phenotypes was examined.

RESULTS

After genotyping, we identified 24 AA, 27 AT and 4 TT subjects. The TT subjects were combined with the AT subjects during the analysis due to its small sample size. No differences were noted in gender distribution, clinical features, and fasting lipid profile between the two genotypic groups (AA vs. AT/TT). The AT/TT group had a higher fasting plasma insulin concentration and a lower %S than the AA group (p = 0.0444 and p = 0.0461, respectively). However, no differences were noted in plasma glucose concentrations and %B. Univariate analysis revealed that this polymorphism explained 7.3% of the variation in %S. Multivariate analysis revealed that the polymorphism was an independent determinant for %S (p = 0.0434) and with body mass index accounted for 28.7% of the variation in %S. In contrast, this polymorphism had no impact on %B.

CONCLUSIONS

The A54T polymorphism at the FABP2 locus is a risk factor for insulin resistance in a Caucasian population.

摘要

背景

脂肪酸结合蛋白2（FABP2）基因座上的A54T多态性被发现与非糖尿病皮马印第安人的胰岛素抵抗有关。为了探究这种关联是否存在于其他人群中，我们进行了一项横断面研究，以检查该多态性在55名糖耐量正常的健康白人受试者胰岛素抵抗中的作用。使用稳态模型评估（HOMA）来评估胰岛素敏感性（%S）和β细胞功能（%B）。通过聚合酶链反应-限制性片段长度多态性分析来确定他们的基因型。检查基因型与表型之间的关系。

结果

基因分型后，我们鉴定出24名AA型、27名AT型和4名TT型受试者。由于TT型受试者样本量小，在分析过程中将其与AT型受试者合并。两组基因型（AA型与AT/TT型）在性别分布、临床特征和空腹血脂谱方面没有差异。AT/TT组的空腹血浆胰岛素浓度高于AA组，%S低于AA组（分别为p = 0.0444和p = 0.0461）。然而，血浆葡萄糖浓度和%B没有差异。单因素分析显示，这种多态性解释了%S变异的7.3%。多因素分析显示，该多态性是%S的独立决定因素（p = 0.0434），与体重指数一起占%S变异的28.7%。相比之下，这种多态性对%B没有影响。