Miranda Neto M H, Molinari S L, Natali M R, Sant'Ana D M
Departamento de Ciências Morfofisiólogicas, Universidade Estadual de Maringá, Brazil.
Arq Neuropsiquiatr. 2001 Mar;59(1):54-9. doi: 10.1590/s0004-282x2001000100012.
We carried out this study with the purpose of analyzing the density of neurons of the myenteric plexus in the mesenteric, intermediate and antimesenteric regions of the ileum of rats. Whole-mounts stained with four different techniques were employed. Through countings under optic microscope in an area of 8.96 mm2 we found the following neuronal means with the techniques of Giemsa, NADH-diaphorase histochemistry, NADPH-diaphorase and acetylcholinesterase, respectively: mesenteric region 2144.40+/-161.05, 1657.80+/-88.23, 473.80+/-19.62, 905.25+/-22.40; intermediate region 1790.60+/-128.24, 1265.20+/-141.17, 371.30+/-27.84, 770.25+/-33.12; antimesenteric region 1647.0+/-76.67, 981.80+/-68.04, 298.50+/-22.75, 704.50+/-69.38. We conclude that there is a variation of neuronal density around the intestinal circumference and this fact independs on the technique used to stain the neurons, and that in a single region the neuronal density varies with the technique employed. We also call attention for the identification of the site were countings were carried out, so that the results of research in this area are not compromised.
我们开展这项研究的目的是分析大鼠回肠肠系膜、中间和反肠系膜区域肌间神经丛的神经元密度。采用了四种不同技术染色的整装标本。通过在光学显微镜下对8.96平方毫米的区域进行计数，我们分别用吉姆萨染色法、NADH-黄递酶组织化学法、NADPH-黄递酶法和乙酰胆碱酯酶法得到了以下神经元平均数：肠系膜区域分别为2144.40±161.05、1657.80±88.23、473.80±19.62、905.25±22.40；中间区域分别为1790.60±128.24、1265.20±141.17、371.30±27.84、770.25±33.12；反肠系膜区域分别为1647.0±76.67、981.80±68.04, 298.50±22.75、704.50±69.38。我们得出结论，肠道周围的神经元密度存在变化，且这一事实与用于染色神经元的技术无关，并且在单个区域内，神经元密度会因所采用的技术而有所不同。我们还提请注意计数进行的部位，以免该领域的研究结果受到影响。
