Cracco C, Filogamo G
Department of Human Anatomy and Physiology, University of Turin, Italy.
Neuroscience. 1994 Jul;61(2):351-9. doi: 10.1016/0306-4522(94)90236-4.
The subpopulation of myenteric neurons able to synthesize nitric oxide was studied quantitatively in the adult rat, using the NADPH-diaphorase histochemical method on whole-mount preparations of distended distal ileum. The spatial density of NADPH-diaphorase-positive myenteric neurons was 2388 +/- 193/cm2 (S.D.; five rats), comprising about 27% of the nerve cell bodies per ganglion. Most neurons were intensely stained and displayed predominantly a Dogiel type I morphology; about 8% of the labelled nerve cells were ovoid neurons, exhibiting a pale cytoplasmic reaction product. The mean somatic size of all NADPH-diaphorase-positive myenteric neurons was 446 +/- 40 microns2, with a mean nuclear size of 96 +/- 18 microns2 (mean values +/- S.D.; five rats). Such values fell exactly within the range of neuronal sizes of the total myenteric population, marked by means of NADH-diaphorase histochemistry. Therefore, the morphometric analysis did not identify any peculiar cell size feature, characterizing this specific nerve cell subpopulation. Thus, the present study provides quantitative data on the size, density and proportion of those myenteric neurons that may synthesize nitric oxide in the distal ileum of the rat.
利用NADPH黄递酶组织化学方法，对成年大鼠扩张回肠远端的整装标本进行研究，定量分析了能够合成一氧化氮的肌间神经元亚群。NADPH黄递酶阳性肌间神经元的空间密度为2388±193个/cm²（标准差；5只大鼠），约占每个神经节神经细胞体的27%。大多数神经元染色强烈，主要呈现Dogiel I型形态；约8%的标记神经细胞为卵圆形神经元，胞质反应产物淡染。所有NADPH黄递酶阳性肌间神经元的平均体细胞大小为446±40μm²，平均核大小为96±18μm²（平均值±标准差；5只大鼠）。这些数值恰好落在通过NADH黄递酶组织化学标记的整个肌间神经群体的神经元大小范围内。因此，形态计量分析未发现任何特殊的细胞大小特征来表征这个特定的神经细胞亚群。因此，本研究提供了关于大鼠回肠远端可能合成一氧化氮的肌间神经元的大小、密度和比例的定量数据。
