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1
Purification of alcohol dehydrogenase from Drosophila by general-ligand affinity chromatography.通过通用配体亲和色谱法从果蝇中纯化乙醇脱氢酶。
Biochem J. 1979 Jun 1;179(3):479-82. doi: 10.1042/bj1790479.
2
Purification and enzyme stability of alcohol dehydrogenase from Drosophila simulans, Drosophila virilis and Drosophila melanogaster adhS.拟果蝇、粗壮果蝇和黑腹果蝇adhS中乙醇脱氢酶的纯化及酶稳定性
Biochem J. 1980 Jul 1;189(1):105-10. doi: 10.1042/bj1890105.
3
Purification and separation of pyridine nucleotide-linked dehydrogenases by affinity chromatography techniques.通过亲和色谱技术纯化和分离吡啶核苷酸连接的脱氢酶。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3450-4. doi: 10.1073/pnas.71.9.3450.
4
Separation of isozymes of horse liver alcohol dehydrogenase and purification of the enzyme by affinity chromatography on an immobilized AMP-analogue.马肝醇脱氢酶同工酶的分离及通过固定化AMP类似物亲和层析法对该酶的纯化。
Biochim Biophys Acta. 1974 Sep 11;364(1):1-8. doi: 10.1016/0005-2744(74)90126-0.
5
The synthesis of three AMP-analogues: N6-(6-aminohexyl)-adenosine 5'-monophosphate, N6-(6-aminohexyl)-adenosine 2',5'-bisphosphate, and N6-(6-aminohexyl)-adenosine 3',5'-bisphosphate and their application as general ligands in biospecific affinity chromatography.三种AMP类似物的合成：N6-(6-氨基己基)-腺苷5'-单磷酸、N6-(6-氨基己基)-腺苷2',5'-二磷酸和N6-(6-氨基己基)-腺苷3',5'-二磷酸及其作为生物特异性亲和色谱通用配体的应用。
Eur J Biochem. 1974 Aug 15;47(1):81-9. doi: 10.1111/j.1432-1033.1974.tb03670.x.
6
Enzyme instability and proteolysis during the purification of an alcohol dehydrogenase from Drosophila melanogaster.从黑腹果蝇中纯化乙醇脱氢酶过程中的酶不稳定性和蛋白水解作用。
Biochem J. 1977 May 1;163(2):317-23. doi: 10.1042/bj1630317.
7
Affinity chromatography on immobilised nucleotides. Some applications to the purification of thermophilic dehydrogenases and kinases.固定化核苷酸亲和层析。在嗜热脱氢酶和激酶纯化中的一些应用。
Eur J Biochem. 1975 Jun 16;55(1):201-9. doi: 10.1111/j.1432-1033.1975.tb02152.x.
8
Preparative purification of homogenous steroid-active isozyme of horse liver alcohol dehydrogenase by affinity chromatography on an immobilized AMP-analog.通过固定化 AMP 类似物亲和层析法对马肝醇脱氢酶的均一甾体活性同工酶进行制备纯化。
Anal Biochem. 1975 Dec;69(2):401-9. doi: 10.1016/0003-2697(75)90142-6.
9
Inosine 5'-monophosphate dehydrogenase of Escherichia coli. Purification by affinity chromatography, subunit structure and inhibition by guanosine 5'-monophosphate.大肠杆菌的肌苷5'-单磷酸脱氢酶。通过亲和色谱法纯化、亚基结构及受鸟苷5'-单磷酸的抑制作用
Biochem J. 1979 Dec 1;183(3):481-94. doi: 10.1042/bj1830481.
10
An affinity adsorbent, 5'-adenylate-aminohexyl-sepharose. II. Purification and characterization of multi-forms of RNase T2.一种亲和吸附剂，5'-腺苷酸-氨基己基-琼脂糖。II.核糖核酸酶T2多种形式的纯化与表征
J Biochem. 1981 Aug;90(2):473-81. doi: 10.1093/oxfordjournals.jbchem.a133494.

引用本文的文献

1
Drosophila lactate dehydrogenase: molecular and genetic aspects.果蝇乳酸脱氢酶：分子与遗传学方面
Biochem Genet. 1982 Dec;20(11-12):1195-1209. doi: 10.1007/BF00498943.
2
Determination of some biochemical and structural features of alcohol dehydrogenases from Drosophila simulans and Drosophila virilis. Comparison of their properties with the Drosophila melanogaster Adhs enzyme.拟暗果蝇和粗壮果蝇乙醇脱氢酶的一些生化及结构特征的测定。将它们的特性与黑腹果蝇Adhs酶进行比较。
Biochem J. 1981 Apr 1;195(1):61-9. doi: 10.1042/bj1950061.
3
Co-purification of coenzyme-dependent enzymes by affinity chromatography.通过亲和色谱法共纯化辅酶依赖性酶。
Mol Cell Biochem. 1983;57(1):27-40. doi: 10.1007/BF00223522.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
GENETIC VARIATION OF ALCOHOL DEHYDROGENASE IN DROSOPHILIA MELANOGASTER.黑腹果蝇中乙醇脱氢酶的遗传变异
Nature. 1964 Nov 28;204:906-7. doi: 10.1038/204906a0.
3
LIVER ALCOHOL DEHYDROGENASE-DPN-PYRAZOLE COMPLEX: A MODEL OF A TERNARY INTERMEDIATE IN THE ENZYME REACTION.肝脏乙醇脱氢酶-DPN-吡唑复合物：酶反应中三元中间体的模型
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
Analysis of bacteriophage T7 early RNAs and proteins on slab gels.平板凝胶上噬菌体T7早期RNA和蛋白质的分析。
J Mol Biol. 1973 Sep 15;79(2):237-48. doi: 10.1016/0022-2836(73)90003-x.
6
Affinity chromatography of macromolecules.大分子的亲和色谱法。
Adv Enzymol Relat Areas Mol Biol. 1972;36:29-89. doi: 10.1002/9780470122815.ch2.
7
8-(6-Aminohexyl)-amino-adenine nucleotide derivatives for affinity chromatography.用于亲和层析的8-(6-氨基己基)-氨基腺嘌呤核苷酸衍生物。
Arch Biochem Biophys. 1974 Aug;163(2):561-9. doi: 10.1016/0003-9861(74)90515-3.
8
General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide.亲和色谱中的通用配体。固定化核苷酸5'-单磷酸腺苷和烟酰胺腺嘌呤二核苷酸结合的酶的辅因子-底物洗脱。
Biochem J. 1972 May;127(4):625-31. doi: 10.1042/bj1270625.
9
Multiple forms of drosophila alcohol dehydrogenase. 3. Conversion of one form to another by nicotinamide adenine dinucleotide or acetone.果蝇乙醇脱氢酶的多种形式。3. 烟酰胺腺嘌呤二核苷酸或丙酮介导的一种形式向另一种形式的转化
Arch Biochem Biophys. 1972 Mar;149(1):22-35. doi: 10.1016/0003-9861(72)90295-0.
10
Alcohol dehydrogenase of Drosophila: interconversion of isoenzymes.果蝇的乙醇脱氢酶：同工酶的相互转化
Science. 1968 Jan 19;159(3812):324-5. doi: 10.1126/science.159.3812.324.

通过通用配体亲和色谱法从果蝇中纯化乙醇脱氢酶。

Purification of alcohol dehydrogenase from Drosophila by general-ligand affinity chromatography.

作者信息

Brown A J, Lee C Y

出版信息

Biochem J. 1979 Jun 1;179(3):479-82. doi: 10.1042/bj1790479.

DOI:10.1042/bj1790479
PMID:112998
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186653/
Abstract

A method for the purification of alcohol dehydrogenase from Drosophila melanogaster is described. The method makes use of 8-(6-aminohexyl)amino-5'-AMP, immobilized on Sepharose 4B, as an affinity ligand. Since alcohol dehydrogenase from Drosophila shows weak affinity for this column, a novel technique was developed to separate alcohol dehydrogenase from both unbound proteins and more strongly bound enzymes. The purification procedure is simple to operate and give a homogeneous preparation in good yield after only three steps.

摘要

描述了一种从黑腹果蝇中纯化乙醇脱氢酶的方法。该方法利用固定在琼脂糖4B上的8-(6-氨基己基)氨基-5'-AMP作为亲和配体。由于来自果蝇的乙醇脱氢酶对该柱的亲和力较弱，因此开发了一种新技术来从未结合的蛋白质和结合更强的酶中分离乙醇脱氢酶。纯化过程操作简单，仅经过三步就能以良好的产率得到均一的制剂。