Stimpfl T, Jurenitsch J, Vycudilik W
Institute of Forensic Medicine, University of Vienna, Austria.
J Anal Toxicol. 2001 Mar;25(2):125-9. doi: 10.1093/jat/25.2.125.
The identification of general unknown poisons in complex biological materials like postmortem blood and tissue is a great challenge for the forensic toxicologist. Therefore, a screening procedure utilizing a semi-automatic work-up with an ASPEC system was developed. A broad range of different compounds can be isolated by using non-selective and generally applicable organic polymeric sorbents such as OASIS HLB or Isolute 101. Because colloidal solutions were applied to these sorbents, the denaturation of proteins, which can result in an irreversible loss of significant compounds by adsorption and occlusion, could be avoided. Because of the process of micellar chromatography followed by liquid-liquid extraction of the crude extract, very clean fractions were obtained from such complex matrices as postmortem blood, liver, and brain samples. High recoveries (72-100%) and good day-to-day relative standard deviations (1-17%) could be achieved with both polymeric sorbents. The procedure paves the way for the identification of general unknown poisons in target organs and is therefore a useful tool in the field of forensic toxicology.
对于法医毒理学家而言，在诸如死后血液和组织等复杂生物材料中鉴定常见未知毒物是一项巨大挑战。因此，开发了一种利用ASPEC系统进行半自动前处理的筛查程序。通过使用非选择性且普遍适用的有机聚合物吸附剂（如OASIS HLB或Isolute 101），可以分离出多种不同的化合物。由于将胶体溶液应用于这些吸附剂，可避免蛋白质变性，而蛋白质变性可能因吸附和堵塞导致重要化合物发生不可逆损失。由于采用了胶束色谱法，随后对粗提物进行液 - 液萃取，从死后血液、肝脏和脑样本等如此复杂的基质中获得了非常纯净的馏分。两种聚合物吸附剂均能实现高回收率（72 - 100%）和良好的日间相对标准偏差（1 - 17%）。该程序为在靶器官中鉴定常见未知毒物铺平了道路，因此是法医毒理学领域的一种有用工具。
