Wang Zhaohong, Zhao Jingzhen, Xing Junbo, He Yi, Guo Dean
School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
J Anal Toxicol. 2004 Mar;28(2):141-4. doi: 10.1093/jat/28.2.141.
A sensitive method for the identification and quantitation of the toxic alkaloids strychnine and brucine from postmortem specimens has been established. After solid-phase extraction using Oasis MCX cartridges the extracts were analyzed by high-performance liquid chromatography with diode-array detection. The limit of detection was 0.5 ng/mL blood for strychnine and brucine, and the limit of quantitation was 5 ng/mL blood for strychnine and brucine. The method was applied for the analysis of blood and gastric contents of a 34-year-old female who died after ingestion of a packet of herbal medicine powder containing the seeds of Strychnos nux-vomica L. Strychnine and brucine were detected in all the samples. The concentration in our case is consistent with that in previous reports.
已建立一种灵敏的方法，用于鉴定和定量死后标本中的有毒生物碱士的宁和马钱子碱。使用Oasis MCX柱进行固相萃取后，提取物通过配有二极管阵列检测的高效液相色谱法进行分析。士的宁和马钱子碱的检测限为0.5 ng/mL血液，定量限为5 ng/mL血液。该方法用于分析一名34岁女性的血液和胃内容物，该女性在摄入一包含有马钱子种子的草药粉后死亡。在所有样本中均检测到士的宁和马钱子碱。我们病例中的浓度与先前报告中的浓度一致。
