GGA蛋白促进网格蛋白在高尔基体反面膜囊上依赖于ARF的募集。

The GGAs promote ARF-dependent recruitment of clathrin to the TGN.

作者信息

Puertollano R, Randazzo P A, Presley J F, Hartnell L M, Bonifacino J S

机构信息

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.

出版信息

Cell. 2001 Apr 6;105(1):93-102. doi: 10.1016/s0092-8674(01)00299-9.

DOI:10.1016/s0092-8674(01)00299-9

PMID:11301005

Abstract

The GGAs constitute a family of modular adaptor-related proteins that bind ADP-ribosylation factors (ARFs) and localize to the trans-Golgi network (TGN) via their GAT domains. Here, we show that binding of the GAT domain stabilizes membrane-bound ARF1.GTP due to interference with the action of GTPase-activating proteins. We also show that the hinge and ear domains of the GGAs interact with clathrin in vitro, and that the GGAs promote recruitment of clathrin to liposomes in vitro and to TGN membranes in vivo. These observations suggest that the GGAs could function to link clathrin to membrane-bound ARF.GTP.

摘要

GGA蛋白构成了一个与模块化衔接子相关的蛋白质家族，它们结合ADP-核糖基化因子（ARF），并通过其GAT结构域定位于反式高尔基体网络（TGN）。在此，我们表明，由于干扰了GTP酶激活蛋白的作用，GAT结构域的结合使膜结合的ARF1.GTP得以稳定。我们还表明，GGA蛋白的铰链区和耳状结构域在体外与网格蛋白相互作用，并且GGA蛋白在体外促进网格蛋白募集到脂质体上，在体内促进其募集到TGN膜上。这些观察结果表明，GGA蛋白可能起到将网格蛋白与膜结合的ARF.GTP连接起来的作用。