Fajardo I, Urdiales J L, Medina M A, Sanchez-Jimenez F
Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Malaga, Campus of Teatinos, 29071 Malaga, Spain.
Biochem Pharmacol. 2001 May 1;61(9):1101-6. doi: 10.1016/s0006-2952(01)00567-6.
Both histamine and polyamines are important for maintaining basophilic cell function and viability. The synthesis of these biogenic amines is regulated by histidine decarboxylase and ornithine decarboxylase, respectively. In other mammalian tissues, an interplay between histamine and polyamine metabolisms has been suspected. In this report, the interplay between histamine and ornithine-derived polyamines was studied in a non-transformed mouse mast cell line (C57.1) treated with phorbol ester and dexamethasone, a treatment previously used to increase histidine decarboxylase expression in mastocytoma and basophilic leukemia. Treatment with phorbol ester and dexamethasone increased histidine decarboxylase expression and intracellular histamine levels in C57.1 mast cells to a greater extent than those found for other transformed basophilic models. The treatment also induced a reduction in ornithine decarboxylase expression, intracellular polyamine contents, and cell proliferation. These results indicate that the treatment induces a co-ordinate response of polyamine metabolism and proliferation in mast cells and other immune-related cells. The decrease in the proliferative capacity of mast cells caused by phorbol ester and dexamethasone was simultaneous to an increase in histamine production. Our results, together with those reported by other groups working with polyamine-treated mast cells, indicate an antagonism between histamine and polyamines in basophilic cells.
组胺和多胺对于维持嗜碱性细胞的功能和活力都很重要。这些生物胺的合成分别由组氨酸脱羧酶和鸟氨酸脱羧酶调节。在其他哺乳动物组织中，人们怀疑组胺和多胺代谢之间存在相互作用。在本报告中，研究了在用佛波酯和地塞米松处理的未转化小鼠肥大细胞系（C57.1）中组胺与鸟氨酸衍生的多胺之间的相互作用，此前曾用这种处理方法来增加肥大细胞瘤和嗜碱性白血病中组氨酸脱羧酶的表达。与其他转化的嗜碱性模型相比，用佛波酯和地塞米松处理能更大程度地增加C57.1肥大细胞中组氨酸脱羧酶的表达和细胞内组胺水平。该处理还导致鸟氨酸脱羧酶表达、细胞内多胺含量和细胞增殖减少。这些结果表明，该处理诱导了肥大细胞和其他免疫相关细胞中多胺代谢和增殖的协同反应。佛波酯和地塞米松导致的肥大细胞增殖能力下降与组胺产生增加同时发生。我们的结果，连同其他研究多胺处理肥大细胞的小组所报告的结果，表明嗜碱性细胞中组胺和多胺之间存在拮抗作用。
