Mathur A, Mathur A K, Gangwar A
Planta Med. 2001 Mar;67(2):181-3. doi: 10.1055/s-2001-11506.
A three-step procedure for complete plantlet regeneration via somatic embryogenesis has been developed in Panax sikkimensis. Somatic embryos (SE) were induced in root callus upon lowering the level of 2,4-D from 1.0 mg/l to 0.25 mg/l in the callusing medium. Maturation of SE occurred on a half-strength MS medium with 0.5 mg/l each of BAP and GA3. An exposure for 15 days of cotyledonary and heart-shaped SE to 1.0 mg/l IBA in liquid shake 1/2 MS medium significantly improved the rate of embryo-to-plantlet conversion and plantlet quality. The procedure has now allowed the retention of high regeneration potential of the root callus for over three years.
锡金人参已开发出一种通过体细胞胚胎发生实现完整植株再生的三步程序。在愈伤组织培养基中将2,4-D的水平从1.0毫克/升降至0.25毫克/升时，根愈伤组织中诱导出体细胞胚（SE）。SE在含有0.5毫克/升BAP和GA3的1/2强度MS培养基上成熟。将子叶形和心形SE在液体振荡的1/2 MS培养基中用1.0毫克/升IBA处理15天，显著提高了胚状体向植株的转化率和植株质量。该程序现已使根愈伤组织的高再生潜力保持了三年多。
