Xu Hong-Yuan, Meng Ai-Dong, He Bing, Zhou Feng-Jue, Lan Tao-Ju, Sha Bo, Xu Hong-Zhang
Agricultural College of Guangxi University, Nanning 530005, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(6):481-3.
To study and improve the tissue culture technology of Panax notoginseng.
Using the callus of leaf blade and leafstalk of P. notogingseng as explants, MS + 2, 4-D 1.5 mg x L(-1) as basal medium, the formation of asexual embryos was induced by added LFS, BA, KT or ZT 0.5 mg x L(-1), and cultured in dark. It cultured then in 2000 lx of illumination for 10-12 h x d(-1) to induce the asexual embryos germinating and developing to be the regenerated-plantlet.
Only the medium added with LFS could induce the formation of asexual embryos, and made it developed to be regenerated-plantlet. The inducing ratio of asexual embryos reached about 85%, and 30% of asexual embryos could grow and develop as robust regenerated-plantlets.
研究并改进三七的组织培养技术。
以三七叶片和叶柄的愈伤组织为外植体，以MS + 2,4 - D 1.5 mg·L⁻¹为基础培养基，添加0.5 mg·L⁻¹的LFS、BA、KT或ZT诱导无性胚形成，并在黑暗中培养。然后在2000 lx光照下培养10 - 12 h·d⁻¹，诱导无性胚萌发并发育成再生植株。
仅添加LFS的培养基能诱导无性胚形成，并使其发育成再生植株。无性胚诱导率达85%左右，30%的无性胚能生长发育成健壮的再生植株。
