Kobayashi M, Horiuchi K, Yoshikawa O, Hirasawa K, Ishii Y, Fujino K, Sugiyama H, Maruhashi K
Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center, Shimizu-Shi, Shizuoka, Japan.
Biosci Biotechnol Biochem. 2001 Feb;65(2):298-304. doi: 10.1271/bbb.65.298.
The reaction mechanism of biodesulfurization was investigated using whole cells of Rhodococcus erythropolis KA2-5-1, which have the ability to convert dibenzothiophene (DBT) into 2-hydroxybiphenyl. The desulfurization patterns of alkyl DBTs were represented by the Michaeis-Menten equation. The values of rate constants, the limiting maximal velocity (Vmax) and Michaelis constant (Km), for desulfurization of alkyl DBTs were calculated. The relative desulfurization activities of various alkyl DBTs were reduced in proportion to the total carbon numbers of alkyl substituent groups. Alkyl DBTs that had a total of six carbons of alkyl substituent groups were not desulfurized. The type or position of alkyl substituent groups had little effect on desulfurization activity. The desulfurization activity of each alkyl DBT, when mixed together, was reduced. This phenomenon was caused by apparent competitive inhibition of substrates. Using the apparent competitive inhibition model, the desulfurization pattern of a multiple components system containing alkyl DBTs was elucidated. This model was also applicable for biodesulfurization of light gas oil.
利用红平红球菌KA2-5-1的全细胞研究了生物脱硫的反应机制，该菌株能够将二苯并噻吩（DBT）转化为2-羟基联苯。烷基DBT的脱硫模式用米氏方程表示。计算了烷基DBT脱硫的速率常数、极限最大速度（Vmax）和米氏常数（Km）值。各种烷基DBT的相对脱硫活性与烷基取代基的总碳原子数成比例降低。烷基取代基总共有六个碳原子的烷基DBT不能脱硫。烷基取代基的类型或位置对脱硫活性影响很小。当混合在一起时，每种烷基DBT的脱硫活性都会降低。这种现象是由底物的明显竞争性抑制引起的。使用明显的竞争性抑制模型，阐明了含有烷基DBT的多组分体系的脱硫模式。该模型也适用于轻质瓦斯油的生物脱硫。
