Yuan S Y, Chang J S, Yen J H, Chang B V
National Institute of Environmental Analysis Environmental Protection Administration, Chungli, Taiwan, ROC.
Chemosphere. 2001 Apr;43(3):273-8. doi: 10.1016/s0045-6535(00)00139-9.
The aerobic biodegradation potential of phenanthrene (a polycyclic aromatic hydrocarbon [PAH]) in river sediment was investigated in the laboratory. Biodegradation rate constants (k1) and half-lives (t1/2) for phenanthrene (5 microg/g) in sediment samples collected at five sites along the Keelung River in densely populated northern Taiwan ranged from 0.12 to 1.13 l/day and 0.61 to 5.78 day, respectively. Higher biodegradation rate constants were noted in the absence of sediment. Two of the sediment samples were capable of biodegrading phenanthrene at initial concentrations 5-100 microg/g; lower biodegradation rates occurred at higher concentrations. Optimal biodegradation conditions were determined as 30 degreesC and pH 7.0. Biodegradation was not significantly influenced by the addition of such carbon sources as acetate, pyruvate, and yeast extract, but was significantly influenced by the addition of ammonium, sulfate, and phosphate. Results show that anthracene, fluorene, and pyrene biodegradation was enhanced by the presence of phenanthrene, but that phenanthrene treatment did not induce benzo[a]pyrene biodegradation during a 12-day incubation period.
在实验室中研究了菲（一种多环芳烃[PAH]）在河流沉积物中的好氧生物降解潜力。在台湾北部人口密集的基隆河沿岸五个地点采集的沉积物样品中，菲（5微克/克）的生物降解速率常数（k1）和半衰期（t1/2）分别为0.12至1.13升/天和0.61至5.78天。在没有沉积物的情况下，观察到更高的生物降解速率常数。其中两个沉积物样品能够在初始浓度为5 - 100微克/克时对菲进行生物降解；在较高浓度下生物降解速率较低。确定最佳生物降解条件为30℃和pH 7.0。添加乙酸盐、丙酮酸盐和酵母提取物等碳源对生物降解没有显著影响，但添加铵、硫酸盐和磷酸盐对生物降解有显著影响。结果表明，在12天的培养期内，菲的存在增强了蒽、芴和芘的生物降解，但菲的处理并未诱导苯并[a]芘的生物降解。
