Umar Zubairu Darma, Aziz Nor Azwady Abd, Zulkifli Syaizwan Zahmir, Mustafa Muskhazli
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Microbiology, Umaru Musa Yar'adua University Katsina, P.M.B. 2218, Katsina State, Nigeria.
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
MethodsX. 2017 Feb 20;4:104-117. doi: 10.1016/j.mex.2017.02.003. eCollection 2017.
Polycyclic Aromatic Hydrocarbons (PAHs) are complex and widely distributed environmental pollutants that can affect living ecosystems. This study was conducted to rapidly degrade phenanthrene and pyrene representing low and high molecular weight of PAHs, respectively. MM045 (KT933253) was identified from used engine oil of contaminated soil. PAHs biodegradation was carried out using 2,6-dichlorophenol indophenol (DCPIP) assay. Biodegradation influencing factors including agitation, temperature, pH, inoculums volume and salinity were enhanced using Response Surface Methodology (RSM) by Central Composite Design (CCD). Phenanthrene and pyrene biodegrading metabolites were identified using gas chromatography mass spectrophotometer (GCMS). •Initial biodegradation indicated 75.2% and 54.3% phenanthrene and pyrene degraded by MM045 within 24 h. After CCD optimisation, 100% degradation was achieved for each of the phenanthrene and pyrene, resulting in the formation of intermediate metabolites.•The identified phenanthrene metabolites were 3,4-dihydroxyphenathrene, phthalic acid, pyruvic acid, acetic acid and oxalic acid. Pyrene intermediates comprised pyrene -4,5-dihydrodiol, 3,4-dihydroxyphenanthrene, phthalic acid, pyruvic acid, acetic acid and lactic acid.• MM045 was proven to be rapid and effective in degrading PAHs within 24 h despite the unavailability of existing literatures on PAHs biodegradation.
多环芳烃（PAHs）是复杂且广泛分布的环境污染物，会影响生物生态系统。本研究旨在分别快速降解代表低分子量和高分子量PAHs的菲和芘。MM045（KT933253）是从受污染土壤的废发动机油中鉴定出来的。使用2,6 - 二氯酚靛酚（DCPIP）测定法进行PAHs生物降解。采用响应面法（RSM）中的中心复合设计（CCD）增强了包括搅拌、温度、pH值、接种量和盐度在内的生物降解影响因素。使用气相色谱 - 质谱仪（GCMS）鉴定菲和芘的生物降解代谢产物。•初始生物降解表明，MM045在24小时内降解了75.2%的菲和54.3%的芘。经过CCD优化后，菲和芘均实现了100%降解，形成了中间代谢产物。•鉴定出的菲代谢产物为3,4 - 二羟基菲、邻苯二甲酸、丙酮酸、乙酸和草酸。芘的中间产物包括芘 - 4,5 - 二氢二醇、3,4 - 二羟基菲、邻苯二甲酸、丙酮酸、乙酸和乳酸。•尽管目前尚无关于PAHs生物降解的文献，但已证明MM045在24小时内能够快速有效地降解PAHs。
