Wechselberger G, Bauer T, Meirer R, Piza-Katzer H, Lille S, Russell R C, Schoeller T
Department of Plastic and Reconstructive Surgery, Leopold-Franzens University Innsbruck, Austria.
Tissue Eng. 2001 Apr;7(2):153-9. doi: 10.1089/107632701300062750.
The purpose of the study was to transplant autologous cultured urothelial cells onto a muscle via fibrin glue as a delivery vehicle to create a vascularized, living matrix lined with urothelium that could subsequently be used for urinary reconstruction. Bladder tissue specimens from male Wistar rats (n = 32; 350--500 g) were harvested for urothelial tissue culture. After 8--10 days when the primary cultures became confluent, the cultured urothelial cells were injected underneath the rectus sheath onto the rectus muscle. As delivery vehicle we compared standard culture media and fibrin glue. At 1- and 4-week intervals following urothelial cell grafting, sections of the muscle were analyzed for urothelial graft take using Hematoxylin & Eosin and immunohistochemical staining. The histology demonstrated viable, multilayered clusters of urothelium cells on the muscle surface only in the group using the fibrin glue delivery vehicle. We conclude that a muscle can be successfully prelaminated with autologously cultured urothelial cells via fibrin glue and has therefore potential for urinary reconstructions.
本研究的目的是通过纤维蛋白胶作为递送载体，将自体培养的尿路上皮细胞移植到肌肉上，以创建一个血管化的、内衬尿路上皮的活基质，随后可用于尿路重建。从雄性Wistar大鼠（n = 32；350 - 500 g）获取膀胱组织标本用于尿路上皮组织培养。在原代培养汇合后的8 - 10天，将培养的尿路上皮细胞注射到腹直肌鞘下方的腹直肌上。作为递送载体，我们比较了标准培养基和纤维蛋白胶。在尿路上皮细胞移植后的1周和4周间隔时间，使用苏木精和伊红以及免疫组织化学染色分析肌肉切片的尿路上皮移植存活情况。组织学显示，仅在使用纤维蛋白胶递送载体的组中，肌肉表面存在存活的、多层的尿路上皮细胞簇。我们得出结论，肌肉可以通过纤维蛋白胶成功地预先内衬自体培养的尿路上皮细胞，因此具有尿路重建的潜力。
