Bach A D, Bannasch H, Galla T J, Bittner K M, Stark G B
Department of Plastic and Hand Surgery, and Valley TEC (Valley Tissue Engineering Center), University of Freiburg-Medical Centre, Freiburg, Germany.
Tissue Eng. 2001 Feb;7(1):45-53. doi: 10.1089/107632701300003287.
In the present study, we have established a technique to create an artificial urethra in a rat animal model by transplantation of in vitro-expanded urothelial cells onto an in vivo-prefabricated tube formation using tissue engineering methods. Urothelial cells from isogenic rats were harvested for culture. A silicon catheter was used to induce a connective tissue capsule-tube formation underneath the abdominal skin. Two weeks later, the cultivated urothelial cells were seeded onto the lumen of this tube using fibrin glue as delivery matrix. The histomorphological and immunohistochemical studies revealed a viable multilayered urothelium, lining the inner surface of the prior formed connective tissue tube-formation 4 weeks after grafting the cells. We have shown that cultured and in vitro-expanded urothelial cells can be successfully reimplanted onto a prefabricated tube-like structure using fibrin glue as a delivery matrix and native cell expansion vehicle. The results suggest that the creation of an artificial urethra may be achieved in vivo using tissue engineering methods, showing potential for urethral reconstruction and providing autologous urothelium for reconstructive surgery in the genitourinary tract.
在本研究中，我们建立了一种技术，通过组织工程方法，将体外扩增的尿路上皮细胞移植到体内预制的管状结构上，在大鼠动物模型中创建人工尿道。采集同基因大鼠的尿路上皮细胞进行培养。使用硅胶导管在腹部皮肤下诱导形成结缔组织囊状管。两周后，使用纤维蛋白胶作为递送基质，将培养的尿路上皮细胞接种到该管的管腔内。组织形态学和免疫组织化学研究显示，在接种细胞4周后，有一层存活的多层尿路上皮，覆盖在先前形成的结缔组织管状结构的内表面。我们已经表明，使用纤维蛋白胶作为递送基质和天然细胞扩增载体，培养并在体外扩增的尿路上皮细胞可以成功地重新植入到预制的管状结构上。结果表明，使用组织工程方法在体内创建人工尿道是可行的，这显示了尿道重建的潜力，并为泌尿生殖道重建手术提供自体尿路上皮。
