Jin Z Y, Gong H
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Academia Sinica, Beijing, China.
Arch Insect Biochem Physiol. 2001 Apr;46(4):175-85. doi: 10.1002/arch.1027.
In Helicoverpa armigera, female moths began to lay eggs on the third day after emergence. Mating stimulated earlier egg maturation/oogenesis (P = 0.002) and oviposition (P << 0.01). We established a suitable bioassay model for the influence of male accessory glands (MAG) on the physiology of virgin females: Crude extracts of MAG (2- to 3-day-old) were injected into 2-day-old virgin females, and the injected females were dissected 20 h after mating. It was shown that crude extracts of MAG stimulated earlier egg maturation (P < 0.001) and oviposition (the oviposition ratio was more than 2 times the ratio of the control). Proteinaceous components in crude extracts purified by fractionation and sub-fractionation in reverse phase high performance liquid chromatography also stimulated earlier egg maturation (P < 0.01) and ovipositon (more than 2 times the ratio of the control), and we called them the oogenesis and ovipostion factors (OOSF). With SDS-PAGE, the molecular mass of the bands from OOSF was estimated to be between 55-66 KD. Arch.
在棉铃虫中，雌蛾羽化后第三天开始产卵。交配促使卵更早成熟/卵子发生（P = 0.002）和产卵（P << 0.01）。我们建立了一个合适的生物测定模型，用于研究雄性附腺（MAG）对未交配雌蛾生理的影响：将2至3日龄MAG的粗提物注射到2日龄未交配雌蛾体内，在交配后20小时解剖注射过的雌蛾。结果表明，MAG粗提物促使卵更早成熟（P < 0.001）和产卵（产卵率是对照组的2倍以上）。通过反相高效液相色谱分级分离和亚分级分离纯化的粗提物中的蛋白质成分也促使卵更早成熟（P < 0.01）和产卵（是对照组的2倍以上），我们将它们称为卵子发生和产卵因子（OOSF）。通过SDS-PAGE，OOSF条带的分子量估计在55 - 66千道尔顿之间。《Archives》
