Angmo Nilza, Sengupta Madhumita, Vimal Neha, Seth Rakesh Kumar
Applied Entomology and Radiation Biology Lab, Department of Zoology, University of Delhi, Delhi 110007, India.
Insects. 2023 Jul 20;14(7):651. doi: 10.3390/insects14070651.
The 'Inherited or F sterility technique' (IS), using sub-sterilized male moths, is a widely proposed pest management tool for Lepidoptera pests in general, and the tobacco cutworm (Fabr.) in particular. However, the multiple mating tendency of female moths and the ejaculate quality of male moths might influence the efficiency of this technique. Reduced ejaculate quality was observed in irradiated males, as evidenced by radiation's impact on certain bio-parameters, such as the weight of the spermatophores and their protein content, sperm count, the molecular expression of the sex peptide receptor () and egg fertility, with a greater impact in F male progeny. During the remating of females with untreated males, irrespective of the irradiation status of the first male, there was an increase in calling behavior, remating propensity and fertility in females, with a larger time gap between consecutive matings. The ability of F male progeny to check remating propensity in females 24 h after the initial mating was lower than that of unirradiated males. Partially sterile (130 Gy) males were as successful as unirradiated males in inducing the level of mating refractoriness in females. Decreased ejaculate quality in F male progeny could be associated with increased female receptivity during remating. Understanding the influence of male moth irradiation, insemination quality and post (initial)-mating intervals on the remating behavior of normal female moths and induced sterility might help in simulation modeling and optimizing IS insect programs.
“遗传或半不育技术”（IS）利用亚不育雄蛾，是一种广泛提议用于防治鳞翅目害虫，尤其是烟草夜蛾（Fabr.）的害虫管理工具。然而，雌蛾的多次交配倾向和雄蛾的射精质量可能会影响该技术的效率。在经辐照的雄蛾中观察到射精质量下降，辐射对某些生物学参数的影响证明了这一点，这些参数包括精包的重量及其蛋白质含量、精子数量、性肽受体（）的分子表达以及卵的受精能力，对F代雄性后代的影响更大。在雌蛾与未处理雄蛾再次交配时，无论第一只雄蛾的辐照状态如何，雌蛾的求偶行为、再次交配倾向和受精能力都会增加，连续交配之间的时间间隔更大。F代雄性后代在初次交配24小时后检查雌蛾再次交配倾向的能力低于未辐照的雄蛾。部分不育（130 Gy）的雄蛾在诱导雌蛾的交配不应性水平方面与未辐照的雄蛾一样成功。F代雄性后代射精质量的下降可能与再次交配期间雌蛾接受性的增加有关。了解雄蛾辐照、授精质量以及初次交配后间隔对正常雌蛾再次交配行为和诱导不育的影响，可能有助于模拟建模和优化IS昆虫防治计划。
